Efficacy of enzyme-linked immunosorbent assay for rapid diagnosis of Bordetella pertussis infection |
| |
| Authors: | A J Lawrence J C Paton |
| |
| Affiliation: | Department of Microbiology, Adelaide Children's Hospital, South Australia. |
| |
| Abstract: | We examined the diagnostic efficacy of an enzyme-linked immunosorbent assay (ELISA) for class-specific antibodies to Bordetella pertussis in acute-phase sera collected from 1,240 patients with suspected pertussis. A total of 833 serum specimens (67%) yielded positive results. The proportion of positive results increased to 77% if a second (convalescent-phase) serum was also tested. By comparison, a bacterial agglutination test for B. pertussis antibodies was positive in only 21% of acute-phase specimens and 50% of paired specimens. The high proportion of acute-phase sera which were ELISA positive indicates that a measurable serologic response has usually occurred by the time the diagnosis is suspected. Thus, the ELISA is potentially the most rapid means of laboratory confirmation of B. pertussis infection. |
| |
| Keywords: | |
|
|
