Selenium and the growth of Haemophilus ducreyi. |
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| Authors: | D A Vanden Berghe |
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| Affiliation: | Laboratory of Microbiology, University of Antwerp, Belgium. |
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| Abstract: | ![]()
One of the growth media in current use for Haemophilus ducreyi comprises Mueller Hinton agar, chocolatised horse blood, serum and IsoVitalex (BBL). For a better understanding of growth factors, attempts were made to simplify this complex medium. The horse blood was replaced by haemin (200 micrograms/ml), the serum by albumin (0.2%), and IsoVitalex was substituted only by L-glutamine 0.01%. Most of the strains grew, but when selenium ions were added in a concentration of 3.25 x 10(-3) micrograms/ml, growth was stimulated and became more luxuriant than growth on conventional media. |
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