| 中国大陆H7N9禽流感病毒流行病学特征及血凝素和神经氨酸酶的分子进化 |
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| 引用本文： | 李鹏媛,徐 浩,顾豪高,马蒙蒙,陆家海,郝元涛,张定梅.中国大陆H7N9禽流感病毒流行病学特征及血凝素和神经氨酸酶的分子进化[J].中山大学学报(医学科学版),2014,35(6):932. |
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| 作者姓名： | 李鹏媛 徐 浩 顾豪高 马蒙蒙 陆家海 郝元涛 张定梅 |
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| 作者单位： | 中山大学公共卫生学院医学统计与流行病学系,广东 广州 510080 |
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| 基金项目： | 国家自然科学青年基金(81201283),国家“十二五”传染病防治科技重大专项(2012ZX10004-213,2012ZX10004-902) |
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| 摘 要： | 摘 要: 【目的】 对中国大陆人感染H7N9禽流感的流行病学特征进行描述分析,并对不同地区?不同时间?不同宿主来源的H7N9禽流感血凝素及神经氨酸酶基因进行分子进化分析,为H7N9禽流感的科学防控提供依据?【方法】 汇总各公开网站发布的人感染H7N9禽流感病例信息,分析人感染H7N9禽流感的流行病学特点;从Genbank筛选H7N9禽流感毒株的核酸序列及氨基酸序列,利用MEGE5.0?Bioedit等软件构建进化树,分析核苷酸及所编码蛋白关键位点的变异情况?【结果】全国共报告451例人感染H7N9禽流感病例,病死率为19.96%,男女性别比约为2.25:1; 60岁以上(含60岁)病例占全部病例的45.76%;浙江,广东,江苏,上海四省市报告病例较多;2013年4月前后和2014年1月至2月为两个发病高峰?进化树显示H7N9禽流感毒株分为美洲来源支和欧亚来源支,分离的时间地点越接近进化关系越近?自韩国2011年野鸟中分离的H7N9毒株的NA基因与中国2013年及2014年分离的H7N9毒株NA基因进化关系较近?广东第一例人H7N9禽流感的NA基因与2013年5月分离自山东环境中的H7N9禽流感的NA基因及2013年4月来自浙江人感染H7N9禽流感的NA基因的同源性较高?所有人感染H7N9毒株NA茎区69~73位缺失了QISNT序列?【结论】 老人及男性是感染H7N9禽流感的高危人群,冬春季是疫情高发期,病例集中分布于东南沿海地区?分子进化分析表明H7N9禽流感毒株有时间和空间的聚集性,时间空间距离越短则进化关系越接近?广东地区H7N9禽流感病毒可能是北方的候鸟迁徙所致,中国H7N9毒株NA片段可能来自韩国禽类携带的毒株?人感染H7N9禽流感病毒的毒力及耐药性尚未发生变异?NA茎区69~73位缺失QISNT序列可能与该病毒具有感染人类的能力有关?
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| 关 键 词： | 禽流感 H7N9 进化 血凝素 神经氨酸酶 |
| 收稿时间： | 2014-08-13 |
Epidemiological Characteristics of Influenza Virus H7N9 and Evolutionary Analysis of Hemagglutinin and Neuraminidase Genes |
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| LI Peng-yuan,XU Hao,GU Hao-gao,MA Meng-meng,LU Jia-hai,HAO Yuan-tao,ZHANG Ding-mei.Epidemiological Characteristics of Influenza Virus H7N9 and Evolutionary Analysis of Hemagglutinin and Neuraminidase Genes[J].Journal of Sun Yatsen University(Medical Sciences),2014,35(6):932. |
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| Authors: | LI Peng-yuan XU Hao GU Hao-gao MA Meng-meng LU Jia-hai HAO Yuan-tao ZHANG Ding-mei |
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| Affiliation: | Department of Medical Statistics and Epidemiology,School of Public Health,Sun Yat-sen University,Guangzhou 510080,China |
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| Abstract: | Abstract:【Objectives】To describe and analyze the epidemiological characteristics of the human infection with avian influenza H7N9 virus in mainland China and conduct a molecular evolution analysis of H7N9 virus hemagglutinin and neuraminidase gene isolated from different areas and hosts at different time, which provides scientific basis for preventing avian influenza A(H7N9) outbreaks. 【Methods】 The cases of the human infection with avian influenza H7N9 virus were collected from websites and the epidemiological characteristics were analyzed. HA and NA gene sequences of avian influenza A H7N9 virus were obtained from Genbank to construct phylogenetic trees with MEGE5.0 software. The mutations of nucleotide sequence and corresponding amino acids were analyzed with Bioedit software. 【Results】 There were 451 cases reported throughout the country. The mortality rate was 19.96%. The ratio of male to female was 2.25:1. The patients older than 60 years (including 60) account for 45.76% of all. There were more patients reported in Zhejiang, Guangdong, Jiangsu and Shanghai than in other provinces. Two incidence peaks occurred: one was around April 2013 and the other was from January 2014 to February 2014. Phylogenetic trees showed that there were two branches of the strain, coming from America and Eurasia, respectively. The closer the time and area of strain separation happened, the higher homology they shared. The NA gene of H7N9 strain isolated from wild birds in South Korean 2011 shared high homology with those isolated from mainland China in 2013 and 2014. NA gene of the first human infection with avian influenza A H7N9 virus in Guangdong shared high homology with ones isolated from Shandong environment in May 2013 and the ones isolated from human patients in Zhejiang in April 2013. There was a 5-amino acid deletions (QISNT) in stalk 67~73 of NA gene of shared by all of the avian influenza A H7N9 virus isolated from human patients. 【Conclusions】 The aged and male are at high risk of being infected with avian influenza A H7N9 virus. Outbreaks usually happens in winter and spring in the southeast coastal areas. There were clusters of molecular evolution in time and space. The outbreak of avian influenza A H7N9 virus in Guangdong may be due to the migration of birds from the north. NA gene of H7N9 stains in China may come from the stains carried on by Korean birds. There was no mutations discovered in the human infection avian influenza A H7N9 virus, while the 69~73 stalk deletion ( QISNT ) may be concerned with the ability of infecting human. |
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| Keywords: | avian influenza virus H7N9 evolution hemagglutinin neuraminidase |
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