| 沉默LIMK1抑制人结肠癌SW480细胞上皮-间质转化 |
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| 引用本文： | 史 玲,' target='_blank'>,苏 坚,' target='_blank'>,孙晓勤,夏 红,刘 芳,苏 琦.沉默LIMK1抑制人结肠癌SW480细胞上皮-间质转化[J].现代肿瘤医学,2020,0(13):2183-2188. |
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| 作者姓名： | 史 玲 ' target='_blank'> 苏 坚 ' target='_blank'> 孙晓勤 夏 红 刘 芳 苏 琦 |
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| 作者单位： | 1.湖南省肿瘤细胞与分子病理学重点实验室，湖南省胃癌研究中心，南华大学肿瘤研究所，湖南 衡阳 421001；2.淄博市妇幼保健院病理科，山东 淄博 255000；3.南华大学附属第二医院病理科，湖南 衡阳 421001 |
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| 基金项目： | National Natural Science Foundation of China(No.81973532，31000629）；国家自然科学基金(编号:81973532，31000629) |
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| 摘 要： | 目的:研究沉默LIMK1对人结肠癌SW480细胞上皮-间质转化（epithelial-mesenchymal transformation，EMT）的影响。方法:RT-PCR、Western blot、免疫荧光与免疫组化检测沉默LIMK1对EMT相关分子表达的影响。裸鼠实验检测沉默LIMK1对移植瘤生长的影响。结果:RT-PCR检测显示，沉默LIMK1可下调Vimentin和上调E-cadherin mRNA表达(P<0.05)。Western blot显示，沉默LIMK1可明显下调SW480细胞Snail及Vimentin蛋白表达和上调E-cadherin表达(P<0.05)。免疫荧光显示，LIMK1沉默细胞Snail与Vimentin阳性信号较对照组明显减弱，而E-cadherin阳性信号显著增强。裸鼠实验结果显示，沉默组肿瘤生长速度较SW480组明显减慢(P<0.05)。沉默组移植瘤瘤重（0.16±0.079）g较SW480组（0.86±0.165）g明显减少，瘤重抑制率为97.7%(P<0.05)。LIMK1沉默组移植瘤较对照组的Ki-67、Vimentin、Snail与CD34阳性表达明显减弱，而E-cadherin表达明显增加。结论:沉默LIMK1可体外抑制人结肠癌SW480细胞EMT。
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| 关 键 词： | LIMK1 人结肠癌SW480细胞 上皮-间质转化 Snail Vimentin E-cadherin |
Silencing LIMK1 inhibits epithelial-mesenchymal transformation of human colon cancer SW480 cells |
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| Shi Ling,' target='_blank'>,Su Jian,' target='_blank'>,Sun Xiaoqin,Xia Hong,Liu Fang,Su Qi.Silencing LIMK1 inhibits epithelial-mesenchymal transformation of human colon cancer SW480 cells[J].Journal of Modern Oncology,2020,0(13):2183-2188. |
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| Authors: | Shi Ling ' target='_blank'> Su Jian ' target='_blank'> Sun Xiaoqin Xia Hong Liu Fang Su Qi |
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| Affiliation: | 1.Cancer Research Institute，Center for Gastric Cancer Research of Hunan Province，Hunan Province Key Laboratory of Cancer Cellular and Molecular Pathology，University of South China，Hunan Hengyang 421001，China；2.Department of Pathology，Maternal and Child Health Hospital of Zibo City，Shandong Zibo 255000，China；3.Department of Pathology，the Second Affiliated Hospital，University of South China，Hunan Hengyang 421001，China. |
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| Abstract: | Objective:To study the effect of LIMK1 silencing on EMT in human colon cancer SW480 cells.Methods:RT-PCR，Western blot，immunofluorescence and immunohistochemistry were used to detect the expression of LIMK1 to EMT-related molecules.The effect of LIMK1 silencing on the growth of transplanted tumor was detected in nude mice.Results:RT-PCR showed that silencing LIMK1 could downregulate Vimentin and upregulate E-cadherin mRNA(P<0.05).Western blot showed that silencing LIMK1 could significantly downregulate the expression of Snail and Vimentin and upregulate the expression of E-cadherin protein in SW480 cells(P<0.05).Immunofluorescence showed that the positive signals of Snail and Vimentin in LIMK1 silent cells were significantly reduced compared with the control group，while the positive signals of E-cadherin were significantly enhanced.The results of nude mice showed that the tumor growth rate in the silent group was significantly slower than that in the SW480 group(P<0.05).The tumor weight of the silent group was (0.16±0.079)g，which was significantly reduced compared with the SW480 group's (0.86±0.165)g，and the inhibition rate of tumor weight was 97.7%(P<0.05).Compared with the control group，the expression of Ki-67，Vimentin，Snail and CD34 in LIMK1 silent group was significantly reduced，while the expression of E-cadherin was significantly increased.Conclusion:Silencing LIMK1 inhibits EMT in human colon cancer SW480 cells in vitro. |
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| Keywords: | LIMK1 human colon cancer SW480 cells EMT Snail Vimentin E-cadherin |
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