| Staurosporine诱导心肌细胞氯通道电流的电生理学特性 |
| |
| 引用本文： | 刘艳,张卫卫,李源,王晓明.Staurosporine诱导心肌细胞氯通道电流的电生理学特性[J].心脏杂志,2009,21(3):292-295. |
| |
| 作者姓名： | 刘艳 张卫卫 李源 王晓明 |
| |
| 作者单位： | 第四军医大学西京医院老年病科，陕西 西安 710032 |
| |
| 基金项目： | 国家自然基金课题项目资助 |
| |
| 摘 要： | 目的 探讨staurosporine（STS）诱导乳鼠心肌细胞凋亡的早期，凋亡性容积减少（AVD）发生时，是否有氯通道电流的产生及其电生理学特性。方法 分别采用低渗组的灌流液和含STS的等渗组灌流液处理原代培养的SD乳鼠心肌细胞，以膜片钳全细胞记录法记录电流。结果 ①以低渗灌流液处理的心肌细胞时，可记录到氯通道电流。该电流呈现类似容积敏感性氯通道电流(volume-sensitive chloride channel current,ICl,Vol)的电生理学特性:即外向整流性、高电位刺激下的时间依赖性失活及对氯通道阻断剂4,4′-异二硫氮氐2,2′-二磺酸(DIDS)的敏感性。②以含4 μmol/L STS的等渗液灌流心肌细胞时，也可记录到类似低渗诱导产生的氯通道电流，具有ICl,Vol的电生理学特性，且用氯通道阻断剂500 μmol/L DIDS后，在+40 mV、+60 mV、+80 mV及+100 mV时，能够明显电压依赖性地阻断该电流。结论 首次应用STS在培养乳鼠心肌细胞中记录到类似容积敏感性氯通道电流。
|
| 关 键 词： | 心肌细胞 staurosporine 容积敏感性氯通道电流 全细胞记录 |
| 收稿时间： | 2008-10-21 |
Electrophysiological characteristics of chloride channel currents in cardiomyocytes induced by staurosporine |
| |
| Abstract: | AIM To explore whether chloride channel currents are present when apoptotic volume decrease (AVD) happens during the early times of neonatal rat cardiomyocytes apoptosis induced by staurosporine (STS), and to study the electrophysiological properties of chloride channel currents. METHODS Primary cultured neonatal rat cardiomyocytes were subjected to hypotonic and isotonic perfusion containing 4 μmol/L STS, respectively. We used whole-cell patch-clamp techniques to record chloride channel currents.RESULTS ①After exposure of cardiac myocytes to hypotonic solution, we recorded chloride channel currents which resembled volume-sensitive chloride channel currents (ICl,Vol): outward rectification, time dependent inactivation, sensitivity to 4,4′-diisothiocya-natostilbene-2,2′-disulfonicacid (DIDS). ②After exposure of cardiac myocytes to 4 μmol/L STS isotonic solution, chloride channel currents showing characterizations of ICl,Vol were also recorded. The currents were voltage dependently blocked by 500μmol/L DIDS at +40mV,+60mV,+80mV,+100mV. CONCLUSION It’s the first time to record similar volume-sensitive outwardly rectifying Cl-currents by STS in primary cultured neonatal rat cardiomyocytes. |
| |
| Keywords: | staurosporine |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《心脏杂志》浏览原始摘要信息 |
|
点击此处可从《心脏杂志》下载全文 |
|
