Ligand activation of overexpressed epidermal growth factor receptors transforms NIH 3T3 mouse fibroblasts. |
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| Authors: | H Riedel S Massoglia J Schlessinger A Ullrich |
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| Affiliation: | Department of Developmental Biology, Genentech, South San Francisco, CA 94080. |
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| Abstract: | The cell surface receptor for the mitogenic peptide epidermal growth factor (EGF) is involved in control of normal cell growth and may play a role in the genesis of human neoplasia such as squamous carcinoma and glioblastoma. Soft-agar growth and focus-formation experiments with NIH 3T3 mouse fibroblasts transfected with an expression plasmid demonstrated the ligand-dependent transforming potential of the human EGF receptor without structural alterations. Activation of overexpressed normal receptor alone appears to be sufficient for transformation of NIH 3T3 cells in vitro. |
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