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| 重组酶在胰岛素生产中的应用 | |
| 引用本文： | 冷春生,崔航,李淑楠. 重组酶在胰岛素生产中的应用[J]. 中国药学, 2014, 23(5): 335-337. DOI: 10.5246/jcps.2014.05.047 |
| 作者姓名： | 冷春生 崔航 李淑楠 |
| 作者单位： | 通化东宝药业股份有限公司,吉林通化134001 |
| 摘 要： | 本文分别测定了重组的与天然的牛胰蛋白酶和羧肽酶B对前胰岛素融合蛋白的裂解活性。水解产物采用HPLC进行分析，发现重组酶具有更强的活性并且副产物少，因此重组酶在胰岛素生产中比天然的牛胰蛋白酶和羧肽酶B具有更好的前景。 |
| 关 键 词： | 重组胰蛋白酶 人胰岛素 重组羧肽酶B 重组酶 重组人胰岛素 |
| 收稿时间： | 2014-01-06 |
Application of recombinant enzymes in the production of human insulin |
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| Chunsheng Leng,Hang Cui,Shunan Li. Application of recombinant enzymes in the production of human insulin[J]. Journal of Chinese Pharmaceutical Sciences, 2014, 23(5): 335-337. DOI: 10.5246/jcps.2014.05.047 | |
| Authors: | Chunsheng Leng Hang Cui Shunan Li |
| Affiliation: | (Tonghua Dongbao Pharmaceutical Co. Ltd., Jilin 134001, China) |
| Abstract: | In the present study, the cleavage activities of both recombinant and bovine enzymes （trypsin and carboxy peptidase B） were determined using proinsulin fusion protein. The proteolysis products were analyzed with HPLC. We found that recombinant enzymes had stronger activities and produced fewer by-products. In addition, recombinant enzymes were more efficient in the production of human insulin than bovine enzymes. |
| Keywords: | Recombinant trypsin Human insulin Recombinant carboxy peptidase B Recombinant enzymes Recombinanthuman insulin |
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