| HIF-1α在压力过负荷大鼠心肌组织中的表达 |
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| 引用本文： | 王 婷,柴春艳,王 甜,徐邦强,赵玉兰,海冰峰.HIF-1α在压力过负荷大鼠心肌组织中的表达[J].心脏杂志,2015,27(6):662-665. |
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| 作者姓名： | 王 婷 柴春艳 王 甜 徐邦强 赵玉兰 海冰峰 |
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| 作者单位： | （1.陕西省人民医院西院一病区，陕西 西安 710068； |
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| 摘 要： | 目的 探讨压力过负荷时大鼠心肌组织低氧诱导因子(HIF)-1α、血管内皮生长因子（VEGF）表达的变化。方法 将45只雄性Wistar大鼠随机分为两组，即假手术组（15只）和模型组（30只）。采用腹主动脉缩窄法建立压力过负荷大鼠模型。分别在6周、12周后，制作两组大鼠的心肌组织切片，检测血流动力学指标以及心肌组织中HIF-1α及其靶基因VEGF的表达。结果 与假手术相比，模型组大鼠心肌组织第6周出现明显肥厚(P<0.05)；心肌组织HIF-1α及靶基因VEGF的表达明显增加，并于第12周出现心力衰竭(P<0.05)；心肌组织中HIF-1α和VEGF的表达仍较假手术组增加(P<0.05)，但较本组第6周大鼠表达明显减少(P<0.05)。结论 压力过负荷造成的大鼠心肌肥厚组织，HIF-1α及靶基因VEGF的表达明显增加，但当出现心力衰竭时，HIF-1α和VEGF的表达则明显减少。
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| 关 键 词： | 低氧诱导因子-1α 压力过负荷 心肌肥厚 心力衰竭 大鼠 |
| 收稿时间： | 2014-10-19 |
Expressive evolution of hypoxia-inducible factor-1α in pressure-overloaded rat hearts |
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| Abstract: | AIM To evaluate the expressive evolution of HIF-1α and VEGF in pressure-overloaded rat hearts. METHODS Forty-five male Wistar rats were randomly divided into sham-operated group (n=15) and model group (n=30). Pressure-overloaded rat heart models were produced by abdominal aortic banding.After 6 weeks and 12 weeks, respectively, myocardium and hemodynamic performance were examined, and the expressions of HIF-1α and VEGF in myocardium of rats were determined. RESULTS After 6 weeks, left ventricular hypertrophy increased in model group compared with sham-operated group (P<0.05). The expressions of HIF-1α and VEGF increased significantly in the model group (P<0.05). After 12 weeks, the expressions of HIF-1α and VEGF in the model group increased compared with those in the sham-operated group (P<0.05), but the expressions decreased significantly compared with those during the 6th week in the same group. CONCLUSION When myocardial hypertrophy caused by pressure-overload exists, HIF-1α and VEGF expressions increase significantly but decrease dramatically when heart failure occurs. |
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