| 地衣抗癌活性部位AMH-T的毒理学研究 |
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| 作者姓名： | 贺云发 贺小琼 姜重阳 王静 胡松杉 税靖霖 |
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| 作者单位： | 1. 广西科技大学医学院 |
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| 基金项目： | 基金: 国家自然科学基金资助项目(30960437); 云南省科技计划项目(2009FXL001); 云南省科技厅联合专项重点项目(2012FB001); |
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| 摘 要： | 目的 对地衣抗癌活性部位AMH-T进行小鼠短期重复剂量给药试验研究,观察AMH-T对小鼠血液常规、脏器系数及脏器组织形态学的影响,为抗癌药物的开发提供安全性依据.方法 将雄性裸鼠随机分为DDP组,DMSO组,AMH-T(50 mg/kg,100 mg/kg,200 mg/kg)组.试验中每4天称量一次动物体重.实验结束时,采用自动血液生化及血细胞分析仪检测血液生化及血液常规指标.解剖并观察主要脏器病变,取心、肝、脾、肾、睾丸等脏器进行称量,计算脏器系数.结果 小鼠短期重复给药试验中,AMH-T能明显升高小鼠血谷丙转氨酶和谷草转氨酶水平(P<0.01),其他血液生化、血常规指标无显著性改变;AMH-T对试验动物体重和心、肝、脾、肾、睾丸的发育无明显影响.结论 AMH-T皮下注射给药具有一定肝毒性,实验剂量范围内未见对小鼠骨髓造血功能具有毒性.
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| 关 键 词： | 地衣 抗癌药物 急性毒性 血常规 脏器系数 |
| 收稿时间： | 2016-05-03 |
Toxicological Study on the Anticancer Bioactive Fraction AMH-T of Lichen |
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| Abstract: | Objective To investigate the effects of anticancer bioactive fraction AMH-T of lichen on blood routine, organ coefficient and organ morphology by carrying out short-term repeated dose toxicity test in rat so as to provide evidence for the development of anticancer drugs. Methods The nude mice were randomly divided into 5groups: DDP group, DMSO group, and three AMH-T groups with the dosage of 50mg/kg, 100mg/kg, and 200 mg/kg respectively. The weights of the mice were recorded every four days. At the end of the experiment, automatic biochemical analyzer and blood cell analyzer were applied to detect the serum biochemical indicators and blood routine indexes. The mice were dissected to observe the pathological changes in main organs. Heart, liver, spleen,kidney and testicle were weighed for organ coefficient calculation. Results In short-term repeated dose toxicity test,AMH-T significantly increased blood ALT and AST levels(P<0.01) and significant change was found in other blood biochemical indexes and blood routine indexes. AMH-T had no obvious effect on weight,development of heart, liver,spleen, kidney and testicle. Conclusion When subcutaneous injection is performed, AMH-T shows hepatotoxicity,but it shows no toxicity on bone marrow hematopoietic function. |
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