| DHA和NS-398联合对人胆管癌QBC939细胞的抑制作用 |
| |
| 引用本文： | 陈云杰,姜海涛,王天飞,任峰,苏辉.DHA和NS-398联合对人胆管癌QBC939细胞的抑制作用[J].中华全科医学,2017,15(11):1860. |
| |
| 作者姓名： | 陈云杰 姜海涛 王天飞 任峰 苏辉 |
| |
| 作者单位： | 宁波市第二医院外二科, 浙江 宁波 315010 |
| |
| 基金项目： | 华美研究基金 (2015HMKY19) |
| |
| 摘 要： | 目的 研究二十二碳六烯酸(docosahexaenoic acid,DHA)和选择性环氧合酶-2(cyclooxygenase-2,COX-2)抑制剂NS-398联合对人胆管癌细胞QBC939的抑制作用。 方法 体外培养人胆管癌QBC939细胞株，分别设立DHA浓度组、NS-398浓度组、二者联合组和空白对照组，即将0、15、30、45、60、75 μg/ml浓度的DHA和0、25、50、100、150、200 μmol/L浓度的NS-398分别联合作用于胆管癌QBC939细胞；应用CCK8法检测其分别对QBC939细胞生长的相对抑制率；采用流式细胞术检测QBC939细胞的凋亡情况；应用Transwell小室检测QBC939细胞的体外侵袭情况。 结果 DHA和NS-398在体外均能够单独抑制QBC939细胞生长，45 μg/ml的DHA联合100 μmol/L的NS-398经24 h作用后，细胞生长抑制率达到90%，实验组与DHA浓度组、NS-398浓度组和空白组差异均有统计学意义(P<0.05)，继续增加2种药物浓度，细胞生长抑制率变化不明显；流式细胞术显示DHA和NS-398联合能明显促进QBC939细胞早期凋亡；15 μg/ml的DHA联合50 μmol/L的NS-398经24 h作用后，对胆管癌细胞生长无明显抑制作用，但QBC939的体外侵袭能力明显降低，与对照组相比差异有统计学意义(P<0.01)。 结论 DHA和NS-398联合能明显抑制胆管癌QBC939细胞生长，促进胆管癌细胞早期凋亡，抑制胆管癌细胞的侵袭，二者联合对胆管癌QBC939细胞生长的抑制作用可能是通过促进细胞早期凋亡实现的。
|
| 关 键 词： | 二十二碳六烯酸 NS-398 胆管癌 凋亡 侵袭 |
| 收稿时间： | 2016-12-05 |
Inhibitory effect of DHA and NS-398 on human cholangiocarcinoma QBC939 cells |
| |
| Affiliation: | Second Department of Surgery, the Second Hospital of Ningbo, Ningbo, Zhejiang 315010, China |
| |
| Abstract: | Objective To investigate the effect of Docosahexaenoic acid(DHA) combined with NS-398 on inhibition of cholangiocarcinoma QBC939 cell lines and its mechanism. Methods The cultured cholangiocarcinoma QBC939 cell line was exposed to 0,15,30,45,60 and 75 μg/ml DHA with 0,25,50,100,150 and 200 μmol/L NS-398,respectively.The absorbance of the QBC939 cells was measured by CCK8 and its growth inhibition ratio was calculated.Flow cytometry and transwell migration assays were applied to detect cell apoptosis and invasion,respectively. Results Exposure to DHA combined with NS-398 could significantly suppress the growth of QBC939 cells(P<0.05).When the concentration of DHA was 45 μg/ml and NS-398 was 100 μmol/L,the relative growth inhibition rate of QBC939 cells was 90%.If two drugs concentrations were increased,the result showed no statistically significant differences.DHA combined with NS-398 could promote QBC939 cells apoptosis at the early stage.When the concentration of DHA was 15 μg/ml and NS-398 was 50 μmol/L,it had no obvious inhibitory effect on QBC939 cell growth,but could obviously inhibit cell invasion,and significant difference was observed between the experimental and control groups(P<0.01). Conclusion DHA combined with NS-398 could promote apoptosis and inhibit growth and invasion of cholangiocarcinoma cells QBC939 in vitro. |
| |
| Keywords: | |
|
| 点击此处可从《中华全科医学》浏览原始摘要信息 |
|
点击此处可从《中华全科医学》下载全文 |
|
