| miR-370-3p对胶质母细胞瘤U87-MG细胞株增殖能力的影响 |
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| 引用本文： | 彭泽生,田道锋,张申起,陈谦学.miR-370-3p对胶质母细胞瘤U87-MG细胞株增殖能力的影响[J].中国临床神经外科杂志,2016,0(4):223-226. |
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| 作者姓名： | 彭泽生 田道锋 张申起 陈谦学 |
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| 作者单位： | 430060 武汉，武汉大学人民医院神经外科（彭泽生、田道锋、张申起、陈谦学）
通讯作者:田道锋，E-mail:tiandaofeng@hotmail.com |
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| 基金项目： | 湖北省自然科学基金(2011CDB493);湖北省卫生厅科研项目(JX6B15) |
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| 摘 要： | 目的 探讨微小RNA-370-3p（miR-370-3p）对人脑胶质瘤细胞系U87-MG增殖能力的影响及其机制。方法 将常规培养的U87-MG细胞分为空白组、无义序列转染组和模拟物转染组，后两组分别转染miRNA无义序列及miR-370-3p模拟物，qRT-PCR法检测其转染效率，免疫印迹法检测转染细胞叉头框蛋白M1（FoxM1）的表达；采用EdU法评估细胞的增殖能力，采用平板克隆形成实验检测细胞的增殖能力。结果 与空白组和无义序列转染组比较，模拟物转染组细胞miR-370-3p表达显著增加（P<0.05），而FoxM1的蛋白表达显著减少（P<0.05）；而且模拟物转染组U87-MG细胞增殖能力及克隆形成率均明显减少（P<0.05）。结论 miR-370-3p能够抑制U87-MG细胞的增殖能力，可能与减少FoxM1的表达有关。
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| 关 键 词： | 胶质瘤 U87-MG细胞 微小RNA miR-370-3p 叉头框蛋白M1 细胞增殖 |
Effects of MircoRNA-370-3p on proliferation of glioma cell line U87-MG and its potential mechanism |
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| PENG Ze-sheng;TIAN Dao-feng;ZHANG Shen-qi;CHEN Qian-xue.Effects of MircoRNA-370-3p on proliferation of glioma cell line U87-MG and its potential mechanism[J].Chinese Journal of Clinical Neurosurgery,2016,0(4):223-226. |
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| Authors: | PENG Ze-sheng;TIAN Dao-feng;ZHANG Shen-qi;CHEN Qian-xue |
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| Affiliation: | Department of Neurosurgery, Renmin Hospital, Wuhan University, Wuhan 430060, China |
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| Abstract: | Objective To explore the effect of MircoRNA (miRNA) -370-3p on the proliferation of U87-MG glioblastoma cells and its mechanism. Methods The cultured U87-MG cells were divided into blank group, mimics transfection group (transfected with Has-miRNA-370-3p mimics) and negative control group (transfected with miRNA-ctrl). Transfections of Has-miRNA-37-3p mimics were mediated by lipofectamine 2000 U87-MG cells. The transfection efficiency were detected by quantative real-time PCR. The expression of Forkhead M1 (FoxM1) protein were investigated by Western blotting 48 h after the transfection. The cell proliferation ability was evaluated by 5-ethynyl-2’-deoxyuridine (EdU) incorporation and colony formation assaies 72 h after the transfection. Results The expression of miRNA-370-3p increased and FoxM1 protein expression decreased significantly in U87-MG cells in the mimics transfection group compared with those in the blank group and negative control group. EdU assay showed that the short-term ability of the U87-MG cells proliferation was significantly lower in the mimics transfected group than those in the other two groups (P<0.05). The colony formation assay showed that the long-term ability of the U87-MG cells proliferation was significantly lower in the mimics transfection group than those in the other two groups (P<0.05). Conclusion It is suggested that upregulation of miRNA-370-3p expression can inhibit proliferation of U87-MG cells, probably by downregulating expression of FoxM1. |
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| Keywords: | Glioma U 87-MG cells MircoRNA-370-3p Proliferation FoxM1 |
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