| 我国近期分离的6株狂犬病病毒的糖蛋白基因序列分析 |
| |
| 引用本文： | 黄思佳,解庭波,吴杰,王月,季雅琦,严家新,徐葛林.我国近期分离的6株狂犬病病毒的糖蛋白基因序列分析[J].国际生物制品学杂志,2013,36(2):57-60. |
| |
| 作者姓名： | 黄思佳 解庭波 吴杰 王月 季雅琦 严家新 徐葛林 |
| |
| 作者单位： | 430060 武汉生物制品研究所有限责任公司基因工程室 |
| |
| 基金项目： | 国家高技术研究发展计划(项目编号：2007AA022402) |
| |
| 摘 要： | 目的 分析我国近期分离的6株代表性狂犬病病毒（rabies virus，RV）的糖（G）蛋白基因序列，了解RV街毒株与疫苗株在核苷酸和氨基酸序列水平的差异。方法 对6个RV街毒株G基因进行逆转录和PCR扩增，经纯化、克隆、测序后获得6条G基因全长序列，采用生物信息学软件对G基因序列进行分析。结果 6个街毒株的G蛋白在核苷酸水平的同源性为97.7％～100％，在氨基酸水平的同源性为97.9%~100%；与CTN-181疫苗株的核苷酸序列同源性最高，为86.7%~87.2%，氨基酸序列同源性为92.7%~93.1%。结论 6个RV街毒株的G蛋白在核苷酸及氨基酸水平上均有变异，但与CTN-181疫苗株关系最近，表明CTN-181疫苗株可能为我国境内流行的狂犬病提供良好的预防作用。
|
| 关 键 词： | 狂犬病病毒 糖蛋白类 基因型 序列分析 DNA |
Analysis on glycoprotein gene sequences of 6 representative rabies viruses recently isolated in China HUANG Si-jia |
| |
| Affiliation: | Center for Rabies Diagnosis, Wuhan Institute of Biological Products Co., Ltd., Wuhan 430060, China |
| |
| Abstract: | Objective To analyze glycoprotein (G) gene sequences of 6 representative rabies viruses recently isolated in China and understand the differences between the street virus strains and vaccine strains at the level of nucleotide and amino acid sequences. Methods The G genes were amplified by PCR and sequenced. The nucleotide and amino acid sequences were analyzed with bioinformatics software. Results The nucleotide sequence homologies of G genes from the 6 street strains were 97.7%-100%, and the deduced amino acid identities were 97.9%-100%. The similarities between these isolates and CTN-181 vaccine strain were 86.7%-87.2% and 92.7%-93.1% at nucleotide and amino acid level, respectively. Conclusions Mutations in both gene sequences and deduced amino acid sequences are found in G proteins of the 6 street virus strains, but these strains are much closer to CTN-181 vaccine strain than other vaccine strains. |
| |
| Keywords: | Rabies virus Glycoproteins Genetype Sequence analysis DNA |
|
| 点击此处可从《国际生物制品学杂志》浏览原始摘要信息 |
|
点击此处可从《国际生物制品学杂志》下载全文 |
