| 气相色谱-串联质谱法测定血清中的16种多环芳烃 |
| |
| 引用本文： | 李静1,' target='_blank'>2 ,黄馨仪1,李卓雅1,赖淼菊1,邹晓莉1. 气相色谱-串联质谱法测定血清中的16种多环芳烃[J]. 现代预防医学, 2022, 0(21): 3974-3978. DOI: 10.20043/j.cnki.MPM.202107116 |
| |
| 作者姓名： | 李静1 ' target='_blank'>2 黄馨仪1 李卓雅1 赖淼菊1 邹晓莉1 |
| |
| 作者单位： | 1.四川大学华西公共卫生学院/四川大学华西第四医院,四川 成都 610041; &2.内江市中医医院 |
| |
| 摘 要： | 目的 建立血清中16种多环芳烃的气相色谱-串联质谱测定方法。方法 血清样经甲醇沉淀蛋白,加入正己烷与二氯甲烷混合溶剂萃取,萃取物氮吹至近干后,正己烷和二氯甲烷混合溶剂复溶,取1 μl上清液进样分析。16种待测多环芳烃经DB-5色谱柱(30 m×0.25 mm,0.25 μm)分离,多反应监测模式监测,内标法定量。结果 本法在1.0 μg/L～2.0×102 μg/L范围内线性良好,方法检出限为4.20×10-2 μg/L～0.27 μg/L,加标回收率为79.7%～108.0%,精密度为2.57%～6.76%。结论 本方法具有灵敏、快速、回收率和重复性好等优点,满足人血清中多种多环芳烃测定的要求。
|
| 关 键 词： | 气相色谱-质谱法 血清 多环芳烃 |
Determination of 16 polycyclic aromatic hydrocarbons in human serum by gas chromatography-tandem mass spectrometry |
| |
| LI Jing,HUANG Xin-yi,LI Zhuo-ya,LAI Miao-ju,ZOU Xiao-li. Determination of 16 polycyclic aromatic hydrocarbons in human serum by gas chromatography-tandem mass spectrometry[J]. Modern Preventive Medicine, 2022, 0(21): 3974-3978. DOI: 10.20043/j.cnki.MPM.202107116 |
| |
| Authors: | LI Jing HUANG Xin-yi LI Zhuo-ya LAI Miao-ju ZOU Xiao-li |
| |
| Affiliation: | Department of Laboratory Technology and Science of Public Health, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China |
| |
| Abstract: | Objective To establish a method for the determination of polycyclic aromatic hydrocarbons(PAHs)in human serum by gas chromatography - tandem mass spectrometry(GC-MS/MS). Methods The protein in serum sample was precipitated with methanol and the PAHs were extracted with a mixed solvent of n-hexane and dichloromethane. After the extract was blown to near dryness with nitrogen, the mixed solvent of n-hexane and dichloromethane was used to reconstitute, and 1 μl of supernatant was taken for analysis. The PAHs were separated with DB-5 chromatographic column(30 m × 0.25 mm, 0.25 μm), and detected with EI ion source, in multi-reaction monitoring mode. The quantification was based on internal standard curve method. Results The method was linear in the range of 1.00 μg/L-2.00×102 μg/L, with the detection limits of 4.20×10-2 μg/L -0.27 μ/L. The recoveries of the method were 79.7% - 108.0%, and the relative standard deviations(RSDs)were 2.57%-6.76%. Conclusion This method is rapid, sensitive, accurate and reproducible, so it is applicable to the determination of PAHs in human serum samples. |
| |
| Keywords: | GC-MS/MS Serum Polycyclic aromatic hydrocarbons |
|
| 点击此处可从《现代预防医学》浏览原始摘要信息 |
|
点击此处可从《现代预防医学》下载免费的PDF全文 |
|
