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Escherichia coli adherence to HEp-2 cells with prefixed cells. |
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| Authors: | H M Zepeda-Lopez and G M Gonzalez-Lugo |
| Abstract: | We describe a new method which uses cold absolute methanol-prefixed cells for adherence of enteropathogenic Escherichia coli to HEp-2 cells. We found that a method using bacteria grown in Penassay broth to 10(6) to 10(7) CFU/ml and incubated with prefixed cells for 3 h at 37 degrees C, showed 100% sensitivity and specificity against a method using live cells. |
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