| LncRNA-ROR抑制结直肠癌细胞转移的作用研究 |
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| 引用本文： | 胡世丰,雷春,施炜,查诚.LncRNA-ROR抑制结直肠癌细胞转移的作用研究[J].蚌埠医学院学报,2020,45(5):569-571,577. |
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| 作者姓名： | 胡世丰 雷春 施炜 查诚 |
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| 作者单位： | 1.铜陵职业技术学院 医学系, 安徽 铜陵 2440612.安徽省铜陵市人民医院 普外科, 244000 |
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| 摘 要： | 目的了解LncRNA-ROR在结直肠癌细胞转移中的作用。方法在前期工作中，已经发现LncRNA-ROR在结直肠癌组织中低表达，并抑制肠癌细胞的增殖。进一步，我们采用Transwell功能研究LncRNA-ROR的过表达对结直肠癌细胞SW620迁移的影响；并采用划痕实验探索LncRNA-ROR过表达后结直肠癌细胞SW620伤痕愈合的变化。结果SW620细胞在ROR过表达后，穿过人工膜的细胞数减少(P＜0.01)；在迁移和侵袭实验中，与对照组比较，SW620 LncRNA-ROR穿过Transwell膜的细胞数减少(P＜0.01)，侵袭能力减弱(P＜0.01)；划痕实验中，划痕48 h后LncRNA-ROR的细胞间间距大于对照组(P＜0.01)。结论LncRNA-ROR抑制结直肠癌细胞的转移。
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| 关 键 词： | 结直肠肿瘤 LncRNA-ROR SW620细胞 转移 |
| 收稿时间： | 2019-08-28 |
Study on the inhibition of LncRNA-ROR cells on colorectal cancer metastasis |
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| Affiliation: | 1.Department of Medicine, Tongling Vocational and Technical College, Tongling Anhui 2440612.Department of General Surgery, Tongling People's Hospital, Tongling Anhui 244000, China |
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| Abstract: | ObjectiveTo investigate the role of LncRNA-ROR cells in metastasis of colorectal cancer.MethodsThe low expression of LncRNA-ROR in colorectal cancer was identified, which could inhibit the proliferation of colorectal cancer cells.The effects of the overexpression of LncRNA-ROR on colorectal cancer SW620 cells metastasis were explored using Transwell assay.The role of the overexpression of LncRNA-ROR on the healing of SW620 cells′ scratch was investigated using the wound healing assay.ResultsAfter LncRNA-ROR was overexpressed in SW620 cells, the number of cells passing through the artificial membrane decreased(P＜0.01).In the migration and invasion experiments, compared with the control group, the number of cells with SW620 LncRNA-ROR passing the Transwell membrane decreased(P＜0.01), and the invasion ability decreased(P＜0.01).In the wound heding assay, the intercellular spacing of LncRNA-ROR after 48 h of scratching was greater than that in control group(P＜0.01).ConclusionsLncRNA-ROR can inhibit the metastasis of colorectal cancer cells. |
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