miR-655在上皮性卵巢癌中的表达研究 |
| |
引用本文: | 马方昊,靳丽杰,叶国柳,杜丹丽.miR-655在上皮性卵巢癌中的表达研究[J].蚌埠医学院学报,2020,45(2):181-184. |
| |
作者姓名: | 马方昊 靳丽杰 叶国柳 杜丹丽 |
| |
作者单位: | 蚌埠医学院第一附属医院 妇产科, 安徽 蚌埠 233004 |
| |
摘 要: | 目的探讨微小RNA(microRNA,miR)-655在上皮性卵巢癌中的表达、作用及其可能的分子机制。方法采用实时定量-PCR检测miR-655在40对上皮性卵巢癌组织和正常组织中的表达,分析miR-655表达水平与临床病理之间的关系。Cell Counting Kit-8(CCK-8)和流式细胞术检测miR-655对卵巢癌细胞增殖及凋亡的影响。Western blotting检测PI3K、AKT和mTOR蛋白的表达。结果miR-655在上皮性卵巢癌组织中的表达量明显低于正常组织(P < 0.01)。miR-655表达水平在不同肿瘤直径、病理分化程度和是否淋巴结转移病人间差异均有统计学意义(P < 0.05~P < 0.01),而不同年龄、CA-125病人间miR-655 mimics表达差异均无统计学意义(P>0.05)。转染miR-655 mimics可明显抑制卵巢癌细胞的增殖并促进细胞的凋亡(P < 0.01);miR-655 mimics转染明显抑制卵巢癌细胞中PI3K、AKT和mTOR活性(P < 0.01)。结论miR-655在卵巢癌组织中明显低表达,miR-655低表达可能与卵巢癌的肿瘤生长和分化程度相关,miR-655可明显抑制卵巢癌细胞的增殖,并促进其凋亡,其在卵巢癌病人中具有一定的靶向治疗价值。
|
关 键 词: | 卵巢肿瘤 miR-655 增殖 凋亡 |
收稿时间: | 2017-10-31 |
Study on the miR-655 expression in epithelial ovarian cancer |
| |
Affiliation: | Department of Gynaecological Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui 233004, China |
| |
Abstract: | ObjectiveTo investigate the expression level, function and possible molecular mechanism of microRNA (miR)-655 in epithelial ovarian cancer.MethodsThe expression levels of miR-655 in 40 cases of epithelial varian cancer tissues and normal tissues were detected using real-time quantitative-PCR, and the relationship between miR-655 expression and clinicopathological features was analyzed.The cell counting Kit-8(CCK-8) and flow cytometry were used to detect the effects of miR-655 on the proliferation and apoptosis of ovarian cancer cells.Western blotting was used to detect the expression of PI3K, AKT and mTOR.ResultsThe expression level of miR-655 in epithelial ovarian carcinoma was significantly lower than that in normal tissues(P < 0.01).The differences of the expression levels of miR-655 among patients with different tumor diameter, degree of pathological differentiation and lymph node metastasis were statistically significant(P < 0.05 to P < 0.01), and the differences of the expression levels of miR-655 among patients with different ages and CA-125 were not statistically significant(P>0.05).The transfection of miR-655 mimics could significantly inhibit the proliferation and promote apoptosis of ovarian cancer cells(P < 0.01).The transfection of MiR-655 mimics significantly could significantly inhibit the expression of PI3K, AKT and mTOR in ovarian cancer cells(P < 0.01).ConclusionsThe low expression of miR-65 in ovarian cancer tissue is significant, which may be related to the growth and differentiation of ovarian cancer.miR-655 can significantly inhibit the proliferation and induce apoptosis of ovarian cancer cells.miR-655 has a certain value of target therapy in ovarian cancer patients. |
| |
Keywords: | |
|
| 点击此处可从《蚌埠医学院学报》浏览原始摘要信息 |
|
点击此处可从《蚌埠医学院学报》下载全文 |
|