Shank1 在肾细胞癌组织中的表达及临床意义 |
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引用本文: | 赵万里,刘胜,刘民,周煦东,孙鹏宇,王宪,杨继宏,马晓丽. Shank1 在肾细胞癌组织中的表达及临床意义[J]. 中国肿瘤临床, 2016, 43(8): 329-333. DOI: 10.3969/j.issn.1000-8179.2016.08.064 |
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作者姓名: | 赵万里 刘胜 刘民 周煦东 孙鹏宇 王宪 杨继宏 马晓丽 |
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作者单位: | 作者单位:①沧州市中心医院泌尿外二科(河北省沧州市061014);②华北油田总医院泌尿外科;③济南市中心医院中心实验室 |
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摘 要: | 目的:检测Shank 家族成员Shank1 在肾细胞癌(renal cell carcinoma,RCC )中的表达,探讨在RCC 癌组织与癌旁组织中Shank1 的表达差异,分析其与RCC 临床病理特征的关系。方法:收集2008年5 月至2014年12月沧州市中心医院与济南市中心医院120 例RCC 术后患者的癌组织与癌旁组织标本,采用免疫组织化学染色及Westernblot检测Shank1 的蛋白表达水平,分析Shank1 表达与RCC 临床病理特征的关系。结果:免疫组织化学结果显示,RCC 的癌组织中Shank1 表达较癌旁组织明显上调,差异具有统计学意义(P < 0.05)。 Westernblot检测发现RCC 的癌组织中Shank1 蛋白表达水平明显高于癌旁组织。对Shank1 表达上调RCC 患者的临床病理特征分析发现,Shank1 在癌组织中的高表达与患者的性别、年龄、肿瘤的大小、TNM 分期无显著性相关(P > 0.05),但与RCC 的不同病理类型显著性相关(P < 0.05)。 结论:Shank1 在RCC 的癌组织中异常表达,并与RCC 的病理类型相关。
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关 键 词: | 肾细胞癌 Shank1? 蛋白表达? 免疫组织化学 |
收稿时间: | 2016-01-18 |
Expression of Shank1 and its clinical significance in renal cancer tissue |
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Affiliation: | 1The Second Department of Urological Surgery, Cangzhou Central Hospital, Cangzhou 061014, China; |
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Abstract: | Objective:To detect the expression of Shank1 protein in renal cell carcinoma (RCC), to investigate its difference between the tumor and carcinoma adjacent tissue, and to analyze its correlation with RCC clinicopathological characteristics and prognosis. Methods:The renal carcinoma and carcinoma adjacent tissues of 120 patients were selected from Cangzhou Central Hospital and Ji'nan Central Hospital from May 2008 to December 2014. The expression level of Shank1 was determined by immunohistochemistry and Western blot. Statistical analysis was performed to determine the relationship between the expression of Shank1 and the clinicopathological features of RCC patients. Results:Results of immunohistochemistry showed that the expression level of Shank1 in renal cancer tissue was significantly higher than that in carcinoma adjacent tissue, and the difference was statistically significant (P<0.05). Western blot results showed that the expression level of Shank1 protein in renal cancer tissue was also significantly higher than in carcinoma adjacent tissue. Correlation analysis found that the high expression level of Shank1 in renal cancer tissue was not significantly related to gender, age, tumor size, and TNM stage, but was significantly associated with the histological differentiation of RCC (P<0.05). Conclusion:Shank1 is abnormal y expressed in RCC renal cancer tissues and is correlated with the histological differentiation of RCC. |
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Keywords: | renal cell carcinoma Shank1 protein expression immunohistochemistry |
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