| CerS2对膀胱癌细胞增殖、迁移及AKT/mTOR信号通路的影响 |
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| 引用本文： | 吕建阳1,李振国1,陈 林1,刘殿刚2. CerS2对膀胱癌细胞增殖、迁移及AKT/mTOR信号通路的影响[J]. 现代肿瘤医学, 2021, 0(22): 3885-3889. DOI: 10.3969/j.issn.1672-4992.2021.22.001 |
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| 作者姓名： | 吕建阳1 李振国1 陈 林1 刘殿刚2 |
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| 作者单位： | 1.北京市第六医院泌尿外科，北京 100007；2.首都医科大学宣武医院普通外科，北京 100053 |
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| 基金项目： | 北京市卫生系统高层次卫生技术人才培养计划项目（编号:2015-3-066） |
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| 摘 要： | 目的:探究CerS2基因在膀胱癌细胞中的作用及其可能的机制。方法:构建pEGFP-C1-CerS2重组表达载体，并转染膀胱癌细胞系T24，使用蛋白质免疫印迹实验检测GFP-CerS2的表达；运用CCK-8法检测细胞增殖活性；运用Annexin Ｖ荧光标记实验检测细胞凋亡水平；分别使用细胞划痕实验及Transwell实验检测细胞迁移能力和侵袭能力；使用蛋白质免疫印迹实验检测细胞中AKT、p-AKT、mTOR及p-mTOR的表达水平。结果:重组GFP-CerS2融合蛋白在T24细胞中可以正常表达，过表达GFP-CerS2显著抑制了膀胱癌细胞的增殖活性，且显著增加了细胞凋亡水平。过表达GFP-CerS2抑制了膀胱癌细胞的迁移和侵袭能力。此外，过表达GFP-CerS2也显著抑制了膀胱癌细胞中AKT和mTOR的磷酸化修饰，但不影响总AKT及mTOR的表达水平。结论:过表达CerS2可以抑制AKT/mTOR信号通路，影响膀胱癌细胞的增殖和凋亡，并抑制细胞的迁移和侵袭能力。
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| 关 键 词： | CerS2 膀胱癌 增殖 凋亡 迁移 AKT/mTOR |
Effects of CerS2 on bladder cancer cell proliferation,migration and AKT/mTOR signaling pathway |
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| LYU Jianyang1,LI Zhenguo1,CHEN Lin1,LIU Diangang2. Effects of CerS2 on bladder cancer cell proliferation,migration and AKT/mTOR signaling pathway[J]. Journal of Modern Oncology, 2021, 0(22): 3885-3889. DOI: 10.3969/j.issn.1672-4992.2021.22.001 |
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| Authors: | LYU Jianyang1 LI Zhenguo1 CHEN Lin1 LIU Diangang2 |
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| Affiliation: | 1.Department of Urology,Beijing Sixth Hospital,Beijing 100007,China;2.General Surgery,Xuanwu Hospital,Capital Medical University,Beijing 100053,China. |
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| Abstract: | Objective:To investigate the role and the possible mechanisms of CerS2 in bladder cancer cells.Methods:The reconstitution expression vectors of pEGFP-C1-CerS2 was constructed,and then transfected into T24 bladder cancer cells.The expression levels of GFP and GFP-CerS2 were examined by immunoblotting assay.The cells proliferation activities were measured by CCK-8 assays.The apoptosis rates were measured by Annexin V fluorescent labeling.The cells migration activities and invasion activities were measured respectively by wound healing assay and Transwell assay.The expression levels of AKT,p-AKT,mTOR and p-mTOR were measured by immunoblotting assay.Results:The reconstituted GFP-CerS2 fusion protein was well expressed in T24 cells.Overexpression of GFP-CerS2 significantly inhibited the proliferation activities in bladder cancer cells,and increased the apoptosis rate.Overexpression of GFP-CerS2 also inhibited the migration and invasion capabilities in bladder cancer cells.In addition,overexpression of GFP-CerS2 remarkably decreased the phosphorylation of AKT and mTOR,but did not affect the total expression of AKT and mTOR.Conclusion:Overexpression of CerS2 suppresses AKT/mTOR signaling pathway,promotes apoptosis,and inhibits the migration and invasion capabilities in bladder cancer cells. |
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| Keywords: | CerS2 bladder cancer proliferation apoptosis migration AKT/mTOR |
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