| 基于Oncomine和TCGA数据库分析E2F5在前列腺癌组织中的表达及临床意义 |
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| 引用本文： | 沈露明,苏健,魏云飞,邓仲磊,朱辰,朱清毅. 基于Oncomine和TCGA数据库分析E2F5在前列腺癌组织中的表达及临床意义[J]. 现代肿瘤医学, 2021, 0(16): 2854-2858. DOI: 10.3969/j.issn.1672-4992.2021.16.020 |
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| 作者姓名： | 沈露明 苏健 魏云飞 邓仲磊 朱辰 朱清毅 |
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| 作者单位： | 江苏省中医院泌尿外科,南京中医药大学附属医院,江苏 南京 210019 |
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| 基金项目： | National Natural Science Foundation of China(No.81772732);国家自然科学基金面上项目(编号:81772732) |
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| 摘 要： | 目的:通过数据挖掘分析E2F5在前列腺癌(PCa)组织中的表达及临床意义.方法:基于GEPIA、On-comine数据库分析E2F5在PCa中的差异性表达及其与预后的关系.高通量基因芯片筛选敲低E2F5差异表达的基因并应用Western blot验证.结果:E2F5 mRNA在PCa组织中表达明显高于正常前列腺组织(P...
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| 关 键 词： | 前列腺癌 E2F5 TCGA数据库 预后 |
Analysis of the expression and clinical significance of E2F5 in prostate cancer based on Oncomine and TCGA database |
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| SHEN Luming,SU Jian,WEI Yunfei,DENG Zhonglei,ZHU Chen,ZHU Qingyi. Analysis of the expression and clinical significance of E2F5 in prostate cancer based on Oncomine and TCGA database[J]. Journal of Modern Oncology, 2021, 0(16): 2854-2858. DOI: 10.3969/j.issn.1672-4992.2021.16.020 |
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| Authors: | SHEN Luming SU Jian WEI Yunfei DENG Zhonglei ZHU Chen ZHU Qingyi |
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| Affiliation: | Department of Urology,Jiangsu Province Hospital of Chinese Medicine,Affiliated Hospital of Nanjing University of Chinese Medicine,Jiangsu Nanjing 210029,China. |
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| Abstract: | Objective:To analyze the expression and clinical significance of E2F5 in prostate cancer based on TCGA database.Methods:The differential expression of E2F5 and the association between E2F5 and clinical prognosis in prostate cancer was analyzed based on the GEPIA and Oncomine databases.RNA-seq analysis was performed to analyze the gene expression profile affected by E2F5 knockdown.Results:The analysis based on the GEPIA showed that the mRNA expression of E2F5 in prostate cancer tissue was significantly higher than that in normal tissue(P＜0.001).The LinkedOmics database showed a positive correlation between the mRNA expression of E2F5 and T stage(P＜0.001)and N stage(P＜0.05).Data acquired from Taylor Prostate 3 cohort via Oncomine database showed that the level of E2F5 was inversely associated with Gleason score(P＜0.05),N stage(P＜0.05)and recurrence status(P＜0.01).Hierarchical clustering revealed a total of 310 upregulated genes and 228 downregulated genes in PC-3 cells transfected with E2F5 siRNA.The KEEG Enrichment analysis showed that E2F5 knockdown primarily affected the signaling pathway gene sets,including the p53 signaling pathway,cell cycle and Wnt signaling pathway.The expression of CDK6 and CDC25A protein decreased in cells transfected with si-E2F5.Conclusion:The expression level of E2F5 mRNA is significantly correlated with with Gleason score,T stage,N stage and recurrence status in patients with prostate cancer.E2F5 may promote the progression of prostate cancer by regulating cell cycle related genes. |
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| Keywords: | prostate cancer E2F5 TCGA database prognosis |
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