| 硫酸酯化茯苓多糖对胃腺癌细胞MKN-45凋亡的影响 |
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| 引用本文： | 李云桥,侯晓华,王艺峰,张俐娜.硫酸酯化茯苓多糖对胃腺癌细胞MKN-45凋亡的影响[J].实用肿瘤学杂志,2012,26(5):423-427. |
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| 作者姓名： | 李云桥 侯晓华 王艺峰 张俐娜 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院老年科(武汉 430022) |
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| 摘 要： | 目的 探讨硫酸酯茯苓多糖(S-PCS3-Ⅱ)诱导胃腺癌细胞凋亡的作用。方法 体外培养低分化胃腺癌细胞株MKN-45,加入不同浓度的硫酸酯茯苓多糖作用于MKN-45细胞不同作用时间后,利用细胞形态学、流式细胞术(FCM)、DNA琼脂糖凝胶电泳、吖啶橙/溴化乙锭双染法、透射电镜等实验方法观察对细胞凋亡的影响。结果 硫酸酯茯苓多糖作用MKN45细胞后,细胞形态学观察见抑制细胞生长,浓度为0.5 g/L的硫酸酯茯苓多糖作用24 h、48 h、72 h后其凋亡率分别为(7.92±0.83)%、(20.68±1.75)%、(37.52±2.47)%,且DNA凝胶电泳出现梯状条带。荧光显微镜和电镜下可见典型的细胞凋亡形态变化。结论 硫酸酯茯苓多糖可诱导胃腺癌细胞凋亡来抑制胃腺癌细胞生长。
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| 关 键 词： | 硫酸酯茯苓多糖 凋亡 MKN-45细胞株 |
| 收稿时间： | 2012-06-04 |
Effects of sulfated derivatives polysaccharides from sclerotium of poria cocos on gastric adenocarcinoma MKN -45 cell apoptosis |
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| LI Yunqiao,HOU Xiaohua,WANG Yifeng,ZHANG Lina.Effects of sulfated derivatives polysaccharides from sclerotium of poria cocos on gastric adenocarcinoma MKN -45 cell apoptosis[J].Journal of Practical Oncology,2012,26(5):423-427. |
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| Authors: | LI Yunqiao HOU Xiaohua WANG Yifeng ZHANG Lina |
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| Affiliation: | Department of Geriatrics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China |
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| Abstract: | Objective To investigate the effect of the sulfated derivatives polysaccharides from sclerotium of Poria cocos （ S - PCS3 - II） on gastric adenoearcinoma MKN - 45 cells apoptosis. Methods The MKN - 45 cell line was cultured in vitro, and was exposed to a range of concentrations of S - PCS3 - II at different time. The effect of apoptosis was observed by cell morphology, flow eytometry, agarose gel DNA electrophoresis, fluorescent microscopy and transmission electronmicroseopy. Results S - PCS3 - II can inhibit the growth of human gastric cancer MKN -45 cells. When MKN -45 cells were treated for 24 h ,48 h ,72 h at 0.5 g/L concentration, the apoptosis rate revealed （7.92 ± 0.83 ） %, （20.68 ± 1.75 ） %, （ 37.52 ± 2.47 ） %, respectively. DNA agarose gel electrophoresis showed the typical DNA ladder of apoptosis. A typical apoptosis morphology was also observed by fluorescent microscopy. A typical apoptosis ultrastructure changes was also observed by transmission electronmicroscopy. Conclusion S - PCS3 - II can inhibit the growth of human gastric cancer MKN - 45cells by inducing gastric cancer cells apoptosis. |
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| Keywords: | Sulfated derivatives polysaccharides from sclerotium of Poria cocos Apoptosis MKN - 45cell strain |
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