| L-精氨酸对严重腹腔感染大鼠肠上皮细胞凋亡的影响 |
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| 引用本文： | 吴承堂,任永锋,刘晋峰,张军花,雷尚通.L-精氨酸对严重腹腔感染大鼠肠上皮细胞凋亡的影响[J].南方医科大学学报,2007,27(11):1728-1730. |
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| 作者姓名： | 吴承堂 任永锋 刘晋峰 张军花 雷尚通 |
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| 作者单位： | 1. 南方医科大学南方医院,普外科,广东,广州,510515
2. 南方医科大学南方医院,手术室,广东,广州,510515 |
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| 摘 要： | 目的 了解L-精氨酸对严重腹腔感染大鼠肠上皮细胞凋亡的影响.方法 18只Wistar大鼠随机分为CLP组(n=6)、ARG组(n=6)和对照组(n=6),CLP组和ARG组均采用大鼠盲肠结扎加穿孔(CLP)制作严重腹腔感染模型;ARG组术后于腹腔注射L-精氨酸(300 mg/kg·d),CLP组术后注射等量生理盐水,对照组仅行剖腹术.各组均于术后24 h取小肠组织,采用原位末端标记法计数各组凋亡细胞.结果 与对照组大鼠比较,CLP组和ARG组小肠黏膜上皮细胞凋亡细胞数量显著升高(P＜0.001);与CLP组比较,ARG组小肠黏膜上皮细胞凋亡细胞数量显著降低(P=0.038).结论 严重腹腔感染可导致大鼠肠黏膜上皮细胞凋亡增多,应用L-精氨酸可减少肠黏膜上皮细胞凋亡.
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| 关 键 词： | 肠上皮细胞 凋亡 腹腔感染 L-精氨酸 |
| 文章编号： | 1673-4254（2007）11-1728-03 |
| 修稿时间： | 2007-03-16 |
L-arginine reduces intestinal epithelial cell apoptosis in rats with severe abdominal infection |
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| WU Cheng-tang,REN Yong-feng,LIU Jin-feng,ZHANG Jun-hua,LEI Shang-tong.L-arginine reduces intestinal epithelial cell apoptosis in rats with severe abdominal infection[J].Journal of Southern Medical University,2007,27(11):1728-1730. |
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| Authors: | WU Cheng-tang REN Yong-feng LIU Jin-feng ZHANG Jun-hua LEI Shang-tong |
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| Affiliation: | 1 Department of General Sugery, 2 Department of Operation, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China |
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| Abstract: | OBJECTIVE: To explore the effect of L-arginine (L-Arg) on intestinal mucosal cell apoptosis in rats with severe abdominal infection. METHODS: Eighteen Wistar rats were randomized into 3 groups, namely the CLP group (n=6) in which the rats were subjected to cecal ligation plus puncture (CLP) to induce severe abdominal infection, L-Arg group (n=6) where the rats received 300 mg/kg peritoneal L-Arg injection following CLP establishment, and the control group (n=6) where the rats underwent ventrotomy only. Intestinal epithelial apoptotic cells were quantified in each group using TUNEL assay 24 h after the operation. RESULTS: Compared with the control group, the rats in CLP and L-Arg groups showed significantly increased number of apoptotic cells in the intestinal epithelium 24 h after the operation (P<0.001). The apoptotic index (AI) in the L-Arg group (18.1-/+2.2) was significantly lower than that in CLP group (20.8-/+2.3, P=0.038). CONCLUSION: Severe abdominal infection results in increased apoptosis of the intestinal epithelial cells in rats, and L-Arg treatment may reduce the cell apoptosis. |
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| Keywords: | intestinal epithelial cell apoptosis abdominal infection L-arginine |
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