Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources. |
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| Authors: | T Adam, G S Gassmann, C Rasiah, U B G bel |
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| Affiliation: | Institut für Medizinische Mikrobiologie und Hygiene, Universit?t Freiburg, Federal Republic of Germany. |
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| Abstract: | A total of 17 B. burgdorferi isolates from various sources were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins, restriction enzyme analysis, Southern hybridization with probes complementary to unique regions of evolutionarily conserved genes (16S rRNA and fla), and direct sequencing of in vitro polymerase chain reaction-amplified fragments of the 16S rRNA gene. Three groups were distinguished on the basis of phenotypic and genotypic traits, the latter traced to the nucleotide sequence level. |
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