| Beagle犬卵母细胞体外成熟研究进展 |
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| 引用本文： | 胡敏华,周治东,倪庆纯,刘运忠. Beagle犬卵母细胞体外成熟研究进展[J]. 实验动物与比较医学, 2017, 25(1): 107-110 |
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| 作者姓名： | 胡敏华 周治东 倪庆纯 刘运忠 |
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| 作者单位： | 广州医药研究总院有限公司实验动物研究开发中心 国家犬类实验动物种子中心, 广州 510240;广州医药研究总院有限公司实验动物研究开发中心 国家犬类实验动物种子中心, 广州 510240;广州医药研究总院有限公司实验动物研究开发中心 国家犬类实验动物种子中心, 广州 510240;广州医药研究总院有限公司实验动物研究开发中心 国家犬类实验动物种子中心, 广州 510240 |
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| 基金项目： | 广州市“珠江科技新星”项目（201610010144）；广州市科技基础条件平台建设项目（201605040005）。 |
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| 摘 要： | 国内实验Beagle犬种质资源保存利用及制备基因修饰人类疾病动物模型，要求有充足的Beagle犬胚胎。目前诱导排卵技术在犬上效果不明显，体内获取犬成熟卵母细胞有困难。同时，尽管科研人员对犬卵母细胞体外成熟培养进行了多方面的尝试，但尚未获得突破，成熟率低，严重制约了其在种质资源保存、基因修饰模型制备及生物医学研究中的应用。本文梳理了不同犬龄及生殖周期、卵母细胞形态与体积及脂滴在Beagle犬卵母细胞体外成熟中的影响，以为其体外成熟寻找新的突破口。
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| 关 键 词： | 犬 疾病模型 卵母细胞 体外成熟 |
| 收稿时间： | 2016-08-09 |
Research progress of in vitro maturation of Beagle dog oocytes |
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| HU Min-hu,ZHOU Zhi-dong,NI Qing-chun and LIU Yun-zhong. Research progress of in vitro maturation of Beagle dog oocytes[J]. Laboratory Animal and Comparative Medicine, 2017, 25(1): 107-110 |
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| Authors: | HU Min-hu ZHOU Zhi-dong NI Qing-chun LIU Yun-zhong |
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| Affiliation: | Research and Development Center of Experimental Animal, Guangzhou General Pharmaceutical Research Institute Co., Ltd., (National Seed Center of Experimental Dogs) Guangzhou 510240, China;Research and Development Center of Experimental Animal, Guangzhou General Pharmaceutical Research Institute Co., Ltd., (National Seed Center of Experimental Dogs) Guangzhou 510240, China;Research and Development Center of Experimental Animal, Guangzhou General Pharmaceutical Research Institute Co., Ltd., (National Seed Center of Experimental Dogs) Guangzhou 510240, China;Research and Development Center of Experimental Animal, Guangzhou General Pharmaceutical Research Institute Co., Ltd., (National Seed Center of Experimental Dogs) Guangzhou 510240, China |
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| Abstract: | Sufficient embryos are needed for the preservation of Beagle dogs germplasm resources and the preparation of gene-modified human disease animal models.Up to now, the induced ovulation technique has no effect on dogs,it is hard to obtain mature oocytes in vivo, although the scientists try a lot in many aspects, but still could not make a breakthrough. The in vitro maturation rate is too low to support the preservation of germplasm resources, application in gene-modified disease models and biomedical research. Aiming to provide useful information on breakthrough in dog oocytes maturation, this review will summarize the effect of different age and reproductive stage,different morphology and size of the oocytes and lipid droplet on the in vitro maturation of dog oocytes. |
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| Keywords: | Beagle dogs Disease model Ooocytes in vitro maturation |
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