| 白色念珠菌经口感染ICR小鼠建立系统性感染模型 |
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| 引用本文： | 罗银珠,潘金春,何丽芳,闵凡贵,叶秋莹,陈锐,吴玉娥,黄韧,张钰. 白色念珠菌经口感染ICR小鼠建立系统性感染模型[J]. 实验动物与比较医学, 2016, 24(6): 591-595 |
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| 作者姓名： | 罗银珠 潘金春 何丽芳 闵凡贵 叶秋莹 陈锐 吴玉娥 黄韧 张钰 |
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| 作者单位： | 广东省实验动物监测所, 广州 510663;广东省实验动物重点实验室, 广州 510663;广东省实验动物监测所, 广州 510663;广东省实验动物重点实验室, 广州 510663;广东省实验动物监测所, 广州 510663;广东省实验动物重点实验室, 广州 510663;广东省实验动物监测所, 广州 510663;广东省实验动物重点实验室, 广州 510663;广东药学院, 广州 510224;广东省实验动物监测所, 广州 510663;广东省实验动物重点实验室, 广州 510663;广东省实验动物监测所, 广州 510663;广东省实验动物重点实验室, 广州 510663;广东省实验动物监测所, 广州 510663;广东省实验动物重点实验室, 广州 510663;广东省实验动物监测所, 广州 510663;广东省实验动物重点实验室, 广州 510663 |
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| 基金项目： | 广东省科技计划项目（编号：2014A010107018）。 |
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| 摘 要： | 目的 探究白色念珠菌（C.albicans）经口感染ICR小鼠建立系统性感染模型，观察C.albicans经黏膜感染后在小鼠体内组织增殖及分布规律。方法 46只ICR雄性小鼠随机分为模型组A（n=20）、模型组B（n=20）、对照组（n=6）。模型组采用棉签法口腔接种C.albicans（7×106cfu/mL），对照组同方法接种等容积生理盐水。模型组A小鼠用于临床、生存、剖检观察试验；模型组B接种后第3、5、7天随机解剖5只小鼠用于组织载菌量和病理检验（对照组每个时间点解剖2只），活菌平板计数法检测小鼠组织载菌量，光镜观察小鼠舌、胃、肝、肾病理组织学变化。结果 模型组小鼠接种后第3天舌面出现明显白斑并随时间加重引起死亡，第5天小鼠死亡率超过50%，第7天死亡率达100%，并能从舌（87.5%）、胃（87.5%）及内脏组织肝（54.5%）、肾（50.5%）、肺（20%）和心脏（4%）中分离到C.albicans，显微镜观察在舌、食道、胃、肝、肾存在菌丝的增殖，对照组未见C.albicans生长，提示模型组小鼠因C.albicans黏膜感染引起小鼠播散性的系统性感染。结论 C.albicans可在一定条件下突破黏膜免疫屏障引起小鼠机会性系统性感染，从而加重感染死亡。
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| 关 键 词： | 白色念珠菌 小鼠口腔 黏膜模型 系统性感染 小鼠 |
| 收稿时间： | 2016-07-08 |
Establishment of a ICR mouse model of systemic C. albicans infection induced by oral inoculation |
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| LUO Yin-zhu,PAN Jin-chun,HE Li-fang,MIN Fan-gui,YE Qiu-ying,CHEN Rui,WU Yu-e,HUANG Ren and ZHANG Yu. Establishment of a ICR mouse model of systemic C. albicans infection induced by oral inoculation[J]. Laboratory Animal and Comparative Medicine, 2016, 24(6): 591-595 |
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| Authors: | LUO Yin-zhu PAN Jin-chun HE Li-fang MIN Fan-gui YE Qiu-ying CHEN Rui WU Yu-e HUANG Ren ZHANG Yu |
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| Affiliation: | Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China;Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663;Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China;Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663;Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China;Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663;Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China;Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663;Guangdong Pharmaceutical University, Guangzhou 510224;Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China;Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663;Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China;Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663;Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China;Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663;Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China;Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663 |
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| Abstract: | Objective To establish a mouse model of systemic C. albicans infection by oral inoculation of the pathogen and observe the proliferation and distribution of C. albicans in vivo tissues.Methods Male ICR mice(n=46)were used as the experiment group(n=40) and blank group (n=6). Cotton swabs with C. albicans were used to infect the mice (7×106 CFU/mL), and the blank group with saline. The mice of the experiment group were randomly divided into two groups:model group A for clinical assessment (n=20) and model group B for tissue fungal burden detection (n=20). Clinical score, survival and autopsy were carried out among the model group A.Five mice were randomly killed from the model group B at 3 d, 5 d and 7 d after infection, respectively (blank group killed 2 mice each time). Microbial load tablet method was used to detect the tissue fungal burdens in different tissues, meanwhile samples of tongue, esophagus, stomach, liver, kidney, lung of infected mice were taken for pathological examination.Results White spot appeared on the surface of tongue since 3 d postinfection and increased with time and finally caused death.The mortality reached over 50% at 5 d. C.albicans was not only detected from the tongue (87.5%), stomach (87.5%), liver (54.5%), kidney (50.5%), lung (20%) and heart (4%), but also was microscopically seen mycelia proliferation in the tongue, stomach, liver, and kidney, yet not seen in the control group, showing that C.albicans caused disseminated systemic infection through mucosal infection in mice.Conclusions C.albicans can induce opportunistic systemic infection by breakthrough the mucosal immune barrier, so as to increase the infection to death. |
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| Keywords: | Candida albicans Oral cavity Mucosal infection Systemic infection Mouse |
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