| CREG在动脉粥样硬化血管中表达的变化 |
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| 引用本文： | 杨桂棠,张娜,段岩,游洋,康建,闫承慧,韩雅玲.CREG在动脉粥样硬化血管中表达的变化[J].心脏杂志,2011,23(5):565-569. |
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| 作者姓名： | 杨桂棠 张娜 段岩 游洋 康建 闫承慧 韩雅玲 |
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| 作者单位： | 沈阳军区总医院心内科，辽宁 沈阳 110840 |
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| 基金项目： | 国家自然科学基金项目资助(30770793,30971218) |
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| 摘 要： | 目的:探讨E1A激活基因阻遏子（CREG）在动脉粥样硬化(AS)发展中的作用。方法: 采用Western blot方法检测AS患者血管中CREG表达的变化。用高脂饲料喂养兔子的方法模拟AS的自热进程，观察血管的形态学变化。用免疫组化染色法检测血管平滑肌细胞(VSMCs)增殖及CREG、SMα-actin和SM MHC表达的变化。结果: 在AS患者的血管中，CREG的表达明显下调。在动物模型中，以高脂饲料喂养兔1个月时，血管内皮出现不光滑、内膜增厚，中膜VSMCs出现表型转化，收缩蛋白SMα-actin和SM MHC的表达开始下降，细胞出现增生，此时CREG的表达也开始下调。以高脂饲料喂养兔2个月时，可见内皮有中断、破损现象，内膜增厚明显，增殖细胞核抗原(PCNA)表达阳性的细胞明显增多，CREG的表达进一步下调，VSMCs中的收缩蛋白也进一步下降。结论: 在AS进程中，血管中膜的VSMCs由收缩表型向合成表型转化，细胞增生活跃、分化减弱，CREG随着AS的发展，在VSMCs中的表达逐步下降，提示CREG作为维持VSMCs分化的蛋白与AS的发生发展密切相关。
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| 关 键 词： | 动脉粥样硬化 E1A激活基因阻遏子 血管平滑肌细胞 兔 |
| 收稿时间： | 2010-11-02 |
Expression of cellular repressor of E1A-stimulated genes in atherosclerotic artery |
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| YANG Gui-tang,ZHANG Na,DUAN Yan,YOU Yang,KANG Jian,YAN Cheng-hui,HAN Ya-ling.Expression of cellular repressor of E1A-stimulated genes in atherosclerotic artery[J].Chinese Heart Journal,2011,23(5):565-569. |
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| Authors: | YANG Gui-tang ZHANG Na DUAN Yan YOU Yang KANG Jian YAN Cheng-hui HAN Ya-ling |
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| Affiliation: | YANG Gui-tang,ZHANG Na,DUAN Yan,YOU Yang,KANG Jian,YAN Cheng-hui,HAN Ya-ling(Department of Cardiology,Shenyang Northern Hospital,Shenyang 110840,Liaoning,China) |
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| Abstract: | AIM:To investigate the effects of cellular repressor of E1A-stimulated genes (CREG) on atherosclerotic lesion formation. METHODS: Western blot was used to detect the expression of CREG in human normal and atherosclerotic arteries from amputation surgery. The morphology of the artery and expression of CREG, smooth muscle α-actin （SMα-actin）and smooth muscle-myosin heavy chain (SM-MHC) on natural atherosclerotic formation were observed in rabbits fed a high-fat diet. RESULTS: In human atherosclerotic artery, the expression of CREG decreased significantly in atherosclerotic artery, accompanied by the decrease of SMα-actin. In rabbit models fed a high-fat diet for 1 month, the endothelium was not smooth and the intima became thicker. The phenotype of vascular smooth muscle cells (VSMCs) changed. Expression of CREG, SMα-actin and SM-MHC decreased and VSMCs proliferated. In rabbit models fed a high-fat diet for 2 months, the endothelium discontinued and the intima became thicker. The number of proliferating cell nuclear antigen (PCNA) positive cells was much higher and the levels of CREG, SMα-actin and SM-MHC protein were lower in VSMCs. CONCLUSION: The phenotype of VSMCs changes from differentiation to synthesis in atherosclerotic formation. CREG expression decreases with the development of atherosclerosis, suggesting that CREG is associated with atherosclerosis. |
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| Keywords: | atherosclerosis E1A-stimulated genes repressor vascular smooth muscle cells rabbit |
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