| PAK5通过上皮-间质转化促进人乳腺癌细胞侵袭 |
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| 引用本文： | 李 洋,邢 瑶,韩馥伊,李 丰. PAK5通过上皮-间质转化促进人乳腺癌细胞侵袭[J]. 现代肿瘤医学, 2018, 0(23): 3705-3708. DOI: 10.3969/j.issn.1672-4992.2018.23.001 |
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| 作者姓名： | 李 洋 邢 瑶 韩馥伊 李 丰 |
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| 作者单位： | 中国医科大学基础医学院分子细胞生物学教研室，教育部医学细胞生物学重点实验室，辽宁 沈阳 110122 |
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| 基金项目： | National Natural Science Foundation of China(No.81602564);国家自然科学基金资助项目（编号:81602564） |
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| 摘 要： | 目的:探讨p21活化激酶-5（p21-activated kinase 5，PAK5）蛋白对乳腺肿瘤细胞侵袭转移的作用机制。方法:观察PAK5过表达对乳腺癌细胞形态的影响，人乳腺肿瘤细胞MCF-7通过慢病毒感染稳定表达Flag-PAK5。然后提取感染细胞的总蛋白进行蛋白免疫印迹检测上皮-间质转化（EMT）标志物上皮-钙黏蛋白（E-cadherin），纤维连接蛋白（Fibronectin）和波形蛋白（vimentin）的表达。最后通过Transwell实验检测过表达PAK5对乳腺癌细胞迁移和侵袭的影响。结果:过表达PAK5蛋白能够使细胞形态由鹅卵石样像梭形变化，下调E-cadherin蛋白，促进乳腺癌细胞迁移和侵袭。结论:PAK5可能参与调节乳腺肿瘤细胞发生上皮－间质转化，促进乳腺肿瘤侵袭转移的发生。
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| 关 键 词： | 乳腺癌 p21活化激酶-5 上皮－间质转化 侵袭转移 |
PAK5 promotes invasion of human breast cancer cells through epithelial-mesenchymal transition |
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| Li Yang,Xing Yao,Han Fuyi,Li Feng. PAK5 promotes invasion of human breast cancer cells through epithelial-mesenchymal transition[J]. Journal of Modern Oncology, 2018, 0(23): 3705-3708. DOI: 10.3969/j.issn.1672-4992.2018.23.001 |
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| Authors: | Li Yang Xing Yao Han Fuyi Li Feng |
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| Affiliation: | Department of Cell Biology，Key Laboratory of Cell Biology，Ministry of Public Health，and Key Laboratory of Medical Cell Biology，Ministry of Education，China Medical University，Liaoning Shenyang 110122，China． |
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| Abstract: | Objective:To investigate the role of p21-activated kinase 5(PAK5)on the metastasis of breast cancer cell lines.Methods:To monitor the effects of PAK5 on cell morphology，human breast cancer cell line MCF7 was stable infection with Flag-PAK5.And whole-cell lysates were extracted，lysates containing equal amounts of protein was detected with Flag，E-cadherin，Fibronectin and vimentin.Then，effects of PAK5 over-expression on the migration and invasion of cultured MCF-7 cells were examined by Transwell migration chambers assay.Results:Overexpression of PAK5 can change cell morphology from cobblestone to fusiform，down-regulate E-cadherin protein and promote migration and invasion of breast cancer cells.Conclusion:PAK5 expression contributes to EMT development in breast cancer cells． |
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| Keywords: | breast cancer p21-activated kinase 5(PAK5) epithelial-mesenchymal transition(EMT) invasion and metastasis |
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