| 中频交变微电流联合替莫唑胺对U251细胞的影响 |
| |
| 引用本文： | 毛 昀,' target='_blank'>,谢 青,张北山,路晓光,' target='_blank'>,梁 衡,' target='_blank'>,陈 辰,' target='_blank'>,唐劲天,李利亚.中频交变微电流联合替莫唑胺对U251细胞的影响[J].现代肿瘤医学,2018,0(16):2494-2498. |
| |
| 作者姓名： | 毛 昀 ' target='_blank'> 谢 青 张北山 路晓光 ' target='_blank'> 梁 衡 ' target='_blank'> 陈 辰 ' target='_blank'> 唐劲天 李利亚 |
| |
| 作者单位： | 1.北京中医药大学，北京 100029；2.中日友好医院中西医结合肿瘤科，北京 100029；3.贵州医科大学附属医院中医科，贵州 贵阳 550001；4.清华大学工程物理系医学物理与工程研究所医疗新技术实验室，北京 100084 |
| |
| 基金项目： | National Natural Science Foundation of China（No.81372412）；国家自然科学基金资助项目（编号:81372412） |
| |
| 摘 要： | 目的:研究中频交变微电流(ACIF)联合替莫唑胺(TMZ)对胶质瘤U251细胞的作用。方法:将对数生长期的U251细胞分别暴露于ACIF组、TMZ组、联合组（ACIF+TMZ）作用下，采用CCK-8法检测细胞存活率，并运用流式细胞技术分析各组细胞的凋亡/死亡和细胞周期状态。结果:本实验将ACIF（50 kHz，150 mA，30 min）作用U251细胞后，细胞存活率为(86.49±0.23)% (P＜0.01)，联合TMZ（IC50）干预后，细胞存活率为（33.28±1.67）%(P＜0.01)；ACIF联合TMZ能够增加细胞凋亡率，阻滞细胞于G2/M期。结论:ACIF对U251细胞的增殖有一定的抑制作用，与TMZ联合应用时能表现出协同抗肿瘤作用。
|
| 关 键 词： | 中频交变微电流 替莫唑胺 U251细胞 细胞周期 细胞凋亡 |
Studies on effects of U251 cells with alternating current at intermediate frequency (ACIF) combined with temozolomide in vitro |
| |
| Mao Yun,' target='_blank'>,Xie Qing,Zhang Beishan,Lu Xiaoguang,' target='_blank'>,Liang Heng,' target='_blank'>,Chen Chen,' target='_blank'>,Tang Jintian,Li Liya.Studies on effects of U251 cells with alternating current at intermediate frequency (ACIF) combined with temozolomide in vitro[J].Journal of Modern Oncology,2018,0(16):2494-2498. |
| |
| Authors: | Mao Yun ' target='_blank'> Xie Qing Zhang Beishan Lu Xiaoguang ' target='_blank'> Liang Heng ' target='_blank'> Chen Chen ' target='_blank'> Tang Jintian Li Liya |
| |
| Affiliation: | 1.Beijing University of Chinese Medicine，Beijing 100029，China;2.Oncology Department of Integrated Traditional Chinese and Western Medicine,China-Japan Friendship Hospital，Beijing 100029，China;3.Traditional Chinese Medicine Department，the Affiliated Hospital of Guizhou Medical University,Guizhou Guiyang 550001,China;4.Institute of Medical Physics and Engineering,Department of Engineering Physics,Tsinghua University，Beijing 100084，China. |
| |
| Abstract: | Objective:To investigate the effect of alternating current at intermediate frequency (ACIF) in combination with temozolomide(TMZ) on glioma U251 cells in vitro.Methods:Cell proliferation in culturing was studied in U251 cell lines exposed to ACIF,TMZ separately and in combinations.The growth rate of U251 cells was evaluated by CCK-8 assay.Cell apoptosis/death and cycle of U251 cells were analyzed by flow cytometry.Results:Survival rate of U251 cells exprosure to ACIF（50 kHz,150 mA,30 min）was (86.49±0.23)%(P＜0.01),but significantly decreased to (33.28±1.67)% (P＜0.01) in combination with temozolomide.Moreover,ACIF in combination with could promote U251 cells apoptosis and induce them to be arrested in G2/M phase.Conclusion:ACIF inhibits U251 cells proliferation and combining with temozolomide can exhibit synergistic antitumor effect. |
| |
| Keywords: | alternating current at intermediate frequency temozolomide U251 cells cell cycle cell apoptosis |
|
| 点击此处可从《现代肿瘤医学》浏览原始摘要信息 |
|
点击此处可从《现代肿瘤医学》下载全文 |
|
