| RGS5在乳腺癌组织和细胞系中的表达和定位 |
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| 引用本文： | 孙舟彤1,何妍彦1,张红艳2. RGS5在乳腺癌组织和细胞系中的表达和定位[J]. 现代肿瘤医学, 2018, 0(23): 3713-3716. DOI: 10.3969/j.issn.1672-4992.2018.23.003 |
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| 作者姓名： | 孙舟彤1 何妍彦1 张红艳2 |
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| 作者单位： | 1.中国医科大学七年制临床医学系，辽宁 沈阳 110122;2.中国医科大学基础医学院细胞生物学教研室，教育部医学细胞生物学重点实验室，辽宁 沈阳 110122 |
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| 基金项目： | National Natural Science Foundation of China(No.81602564，31401115);国家自然科学基金资助项目（编号:81602564，31401115）；辽宁省大学生创新创业训练计划资助项目（编号:201710159000045） |
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| 摘 要： | 目的:探讨G蛋白信号转导调节蛋白5（RGS5）在人乳腺癌细胞系中的表达。方法:提取人正常乳腺上皮细胞HBL100，人乳腺癌细胞系MCF-7，ZR-75-1，ZR-75-30，MDA-MB-231，HCC1937的总蛋白和总RNA，提取细胞的总蛋白和总RNA进行蛋白免疫印迹和实时定量PCR检测RGS5的表达。共聚焦激光扫描显微镜观察RGS5胞内定位；免疫组化检测RGS5在乳腺肿瘤组织中表达情况。结果:MDA-MB-231细胞系中RGS5基因表达上调，MCF7和ZR-75-1细胞系中RGS5蛋白表达提高。结论:RGS5在乳腺癌细胞的高表达可能参与其生长和转移，揭示RGS5可能是人乳腺肿瘤的治疗靶点。
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| 关 键 词： | RGS5 乳腺癌 蛋白表达 |
RGS5 expression and localization in breast cancer tissues and cell lines |
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| Sun Zhoutong1,He Yanyan1,Zhang Hongyan2. RGS5 expression and localization in breast cancer tissues and cell lines[J]. Journal of Modern Oncology, 2018, 0(23): 3713-3716. DOI: 10.3969/j.issn.1672-4992.2018.23.003 |
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| Authors: | Sun Zhoutong1 He Yanyan1 Zhang Hongyan2 |
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| Affiliation: | 1.Major of Clinical Medicine，China Medical University，Liaoning Shenyang 110122，China；2.Department of Cell Biology，Key Laboratory of Cell Biology，Ministry of Public Health and Key Laboratory of Medical Cell Biology，Ministry of Education，College of Basic Medical Science，China Medical University，Liaoning Shenyang 110122，China． |
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| Abstract: | Objective:To investigate the expression of RGS5 protein and gene in human breast cancer cell lines． Methods:Western blot analysis was used to determine RGS5 protein expression in breast cancer cell lines，Real-time PCR analysis for exogenous RGS5 expression from breast cancer cell lines．Results:RGS5 gene was higer expressed in MDA-MB-231 than the other cell lines and RGS5 protein was higher expressed in MCF7 and ZR-75-1.Conclusion:RGS5 protein was expressed in breast cancer cell lines，which may be one of the mechanisms that attribute to generation and metastasis of cancer cells． |
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| Keywords: | RGS5 breast cancer protein expression |
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