| 通过新生树鼩乙型肝炎病毒感染模型动态观察宿主体内TLR-4和IL-6的表达 |
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| 引用本文： | 范倪,陆玉秀,唐艳萍,李科志,何盼,曹骥,陆玉雷,林有智,杨春.通过新生树鼩乙型肝炎病毒感染模型动态观察宿主体内TLR-4和IL-6的表达[J].中国癌症防治杂志,2018,10(2):95-99. |
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| 作者姓名： | 范倪 陆玉秀 唐艳萍 李科志 何盼 曹骥 陆玉雷 林有智 杨春 |
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| 作者单位： | 广西医科大医学附属肿瘤医院实验研究部；广西医科大学研究生院 |
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| 基金项目： | 国家自然科学基金资助项目（81460418） |
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| 摘 要： | 目的 通过观察接种乙型肝炎病毒（hepatitis B virus，HBV）后新生树鼩体内细胞因子TLR-4和IL-6的变化，初步探讨HBV感染过程中宿主体内的免疫反应。方法 将31只树鼩幼崽分为实验组（n=25）和对照组（n=6）。实验组接种人HBV，接种后第8周至第24周定期检测树鼩外周血中乙肝表面抗原（HBsAg）和HBV- DNA，根据检测结果将实验组分为慢性感染组7只（血清HBsAg或HBV-DNA持续阳性时间达到24周）和疑似慢性感染组5只（血清HBsAg或HBV-DNA不连续出现2次或2次以上阳性），其余13只HbsAg和HBV-DNA均阴性，不纳入研究；对照组不做任何处理。采用双抗体夹心法检测树鼩外周血TLR-4、IL-6蛋白表达水平，RT-PCR法检测树鼩肝组织中TLR-4、IL-6 mRNA的表达水平。 结果 观察期间内，慢性感染组和疑似慢性感染组外周血TLR-4与IL-6蛋白的表达均高于对照组（P＜0.05）；肝组织中IL-6的mRNA表达水平高于对照组（P＜0.05）；慢性感染组TLR-4 mRNA表达水平高于对照组（P＜0.05）。结论 TLR-4、IL-6在树鼩HBV感染模型中表达升高，可能在HBV感染过程中起重要作用。
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Detecting changes in TLR-4 and IL-6 during hepatitis B virus infection in a tree shrew model |
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| Fan Ni,Lu Yuxiu,Tang Yanping,Li Kezhi,He Pan,Cao Ji,Lu Yulei,Lin Youzhi,Yang Chun.Detecting changes in TLR-4 and IL-6 during hepatitis B virus infection in a tree shrew model[J].Chinese Journal of Oncology Prevention and Treatment,2018,10(2):95-99. |
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| Authors: | Fan Ni Lu Yuxiu Tang Yanping Li Kezhi He Pan Cao Ji Lu Yulei Lin Youzhi Yang Chun |
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| Abstract: | Objective To detect changes in TLR-4 and IL-6 in tree shrews infected by hepatitis B virus. Methods A total of 25 tree shrews were inoculated with human hepatitis B virus （HBV） and then divided into an HBV-infected group，suspected HBV-infected group and control group based on detection of the HBV marker HBsAg or HBV DNA using a photochemical method or fluorescence- probe PCR. HBV-infected animals showed persistent positive results for HBsAg or HBV DNA，while the suspected HBV-infected group showed two or more positive results for HBsAg or HBV DNA. Levels of TLR-4 and IL-6 in peripheral blood and liver tissues were measured using，respectively，enzyme-linked immunosorbent assay （ELISA） and hematoxylin- eosin staining. Levels of TLR-4 and IL-6 mRNA in liver tissue of all three groups were measured using real-time fluorescence quantitative PCR. All assays were performed between post-natal weeks 8 and 24. Results Seven animals were assigned to the HBV-infected group，while five were assigned to the suspected HBV-infected group； all control animals showed negative results. Levels of TLR-4 in the peripheral blood of infected and suspected infected animals were significantly higher than in control animals （P＜0.05）at weeks of 8，12，16，20，and 24. Levels were not significantly different between the infected and suspected infected groups. Similar results were observed for levels of IL-6 in peripheral blood. Levels of TLR-4 and IL-6 mRNA in liver tissue were significantly higher in the infected and suspected infected groups than in the control group（P＜0.05）. Conclusion Levels of TLR-4 and IL-6 are elevated in tree shrews following infection with HBV virus，and these signaling molecules may play an important role in the infection process. |
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| Keywords: | Hepatitis B virus TLR-4 IL-6 Tree shrew Immune response |
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