| Ghrelin促进乳腺癌细胞MDA-MB-231增殖的分子机制 |
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| 引用本文： | 朱建华,贾 珉,王月芹,黄 炎. Ghrelin促进乳腺癌细胞MDA-MB-231增殖的分子机制[J]. 现代肿瘤医学, 2018, 0(10): 1488-1491. DOI: 10.3969/j.issn.1672-4992.2018.10.002 |
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| 作者姓名： | 朱建华 贾 珉 王月芹 黄 炎 |
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| 作者单位： | 华中科技大学同济医学院附属武汉市第一医院临床免疫学实验室,湖北 武汉 430022 |
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| 基金项目： | National Natural Science Foundation of China（No.81502222）；国家自然科学基金（编号:81502222）;湖北省自然科学基金（编号:2013CFB370）；武汉市卫计委课题（编号:武卫2007-43） |
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| 摘 要： | 目的:探讨生长激素释放肽（Ghrelin）促进乳腺癌细胞MDA-MB-231增殖的分子机制。方法:乳腺癌细胞MDA-MB-231经Ghrelin、Ghrelin受体（growth hormone secretagogue receptor，GHSR)抑制剂［D-Lys3］-GHRP-6或哺乳动物雷帕霉素靶蛋白(mammalian target of Rapamycin，mTOR)抑制剂雷帕霉素（Rapamycin）处理后，MTT或BrdU实验检测MDA-MB-231细胞的增殖能力；Western blot检测MDA-MB-231细胞GHSR表达及mTOR、p70S6K和S6磷酸化水平。结果:增殖实验结果表明Ghrelin增强MDA-MB-231细胞增殖能力；Western blot检测发现Ghrelin激活MDA-MB-231细胞mTOR、p70S6K和S6磷酸化，［D-Lys3］-GHRP-6或Rapamycin消除Ghrelin促进MDA-MB-231细胞增殖效应，同时抑制Ghrelin诱导的mTOR、p70S6K和S6磷酸化。结论:Ghrelin通过与GHSR结合激活MDA-MB-231细胞mTOR/p70S6K/S6信号途径促进MDA-MB-231细胞增殖。
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| 关 键 词： | MDA-MB-231细胞 生长激素释放肽（Ghrelin） 增殖 GHSR mTOR |
The molecular mechanism of Ghrelin induces breast cancer cell MDA-MB-231 proliferation |
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| Zhu Jianhua,Jia Min,Wang Yueqin,Huang Yan. The molecular mechanism of Ghrelin induces breast cancer cell MDA-MB-231 proliferation[J]. Journal of Modern Oncology, 2018, 0(10): 1488-1491. DOI: 10.3969/j.issn.1672-4992.2018.10.002 |
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| Authors: | Zhu Jianhua Jia Min Wang Yueqin Huang Yan |
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| Affiliation: | Laboratory of Clinical Immunology,Wuhan No.1 Hospital,Tongji Medical College,Huazhong University of Science and Technology(HUST),Hubei Wuhan 430022,China. |
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| Abstract: | Objective:To investigate the molecular mechanism of Ghrelin induces breast cancer cell MDA-MB-231 proliferation.Methods:Human breast cancer cell MDA-MB-231 were treated with Ghrelin,［D-Lys3］-GHRP-6 and Rapamycin.The proliferation of MDA-MB-231 was determined by MTT assay and BrdU assay.The expression of GHSR and phosphorylation of mTOR,p70S6K,S6 was detected by Western blot.Results:The proliferation of MDA-MB-231 cells was increased by Ghrelin.Western blot showed that GHSR was expressed on MDA-MB-231 cells and Ghrelin induced rapid phosphorylation of mTOR,p70S6K and S6,which was lower in D-L-GHRP group compared with the Ghrelin group.Conclusion:The results demonstrate that Ghrelin induces MDA-MB-231 cells proliferation through binding to GHSR and activating mTOR/p70S6K/S6 signaling pathway. |
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| Keywords: | MDA-MB-231 Ghrelin proliferation GHSR mTOR |
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