| L1CAM在甲状腺乳头状癌组织及细胞中的表达及与临床病理特征的关系 |
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| 引用本文： | 樊新龙,闫晓菲,张 睿,刘 放,赵月皎,石 刚.L1CAM在甲状腺乳头状癌组织及细胞中的表达及与临床病理特征的关系[J].现代肿瘤医学,2018,0(23):3752-3756. |
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| 作者姓名： | 樊新龙 闫晓菲 张 睿 刘 放 赵月皎 石 刚 |
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| 作者单位： | 1.中国医科大学肿瘤医院 辽宁省肿瘤医院头颈外科；2.结直肠外科，辽宁 沈阳 110042 |
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| 基金项目： | National Natural Science Foundation of China(No.81672427);国家自然科学基金资助项目（编号:81672427）；辽宁省博士科研启动基金（编号:201601417） |
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| 摘 要： | 目的:检测甲状腺乳头状癌组织及细胞株中L1CAM表达，探讨其与临床病理特征的相关性及意义。方法:选取手术切除甲状腺乳头状癌组织、对应的癌旁组织及正常甲状腺组织标本各32例，甲状腺癌细胞株及正常甲状腺细胞株。采用Western blot及qRT-PCR检测组织及细胞株中L1CAM的表达，观察其与肿瘤部位、直径、数目、分化程度、淋巴结转移及AJCC分期等临床病理参数的关系。结果:L1CAM蛋白及mRNA在甲状腺乳头状癌组织中表达高于癌旁甲状腺组织，在正常甲状腺组织无表达（P<0.05）；甲状腺乳头状癌组织中L1CAM蛋白水平与肿瘤直径、肿瘤数目、淋巴结转移、AJCC分期具有相关性（P均＜0.05），肿瘤直径大于1 cm、肿瘤多发、淋巴结转移及AJCC分期Ⅲ-Ⅳ的甲状腺癌组织中L1CAM表达量高；L1CAM蛋白及mRNA在BHT101、B-CPAP甲状腺癌细胞株中均有表达，在正常甲状腺上皮细胞NTHY-ORI 3-1无表达，在BHT101中高于其他细胞株（P<0.05）。结论:L1CAM在甲状腺乳头状癌组织及细胞中表达上调，与甲状腺乳头状癌发展及转移恶性临床病理特征相关。
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| 关 键 词： | 甲状腺癌 L1CAM基因 BHT101 |
Expression of L1CAM in thyroid papillary cancer tissues and cells and the association with clinicopathological characteristics |
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| Fan Xinlong,Yan Xiaofei,Zhang Rui,Liu Fang,Zhao Yuejiao,Shi Gang.Expression of L1CAM in thyroid papillary cancer tissues and cells and the association with clinicopathological characteristics[J].Journal of Modern Oncology,2018,0(23):3752-3756. |
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| Authors: | Fan Xinlong Yan Xiaofei Zhang Rui Liu Fang Zhao Yuejiao Shi Gang |
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| Affiliation: | 1.Department of Head and Neck Surgery；2.Department of Colorectal Surgery，Cancer Hospital of China Medical University，Liaoning Cancer Hospital & Institute，Liaoning Shenyang 110042，China. |
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| Abstract: | Objective:To detect the expression of L1CAM in thyroid papillary cancer tissues and cell lines at protein and mRNA level，and discuss the association with clinicopathological characteristics.Methods:Thirty-two paired samples of thyroid papillary cancer tissues，para-cancer tissues，normal tissues，thyroid cancer cell lines and normal thyroid cell lines were selected and L1CAM expression was detected by Western blot and quantitative real-time polymerase chain reaction（qRT-PCR）.The association between L1CAM protein levels and tumor location，diameter，number，differentiation，lymph node metastasis and AJCC stage was analyzed.Results:Expression of L1CAM protein and mRNA in thyroid papillary cancer tissues was higher than in adjacent normal thyroid tissues （P<0.05）.L1CAM was not expressed in normal thyroid tissues.L1CAM protein level in thyroid tissues was correlated with tumor diameter，tumor number，lymph node metastasis，AJCC stage（P<0.05）.L1CAM was high expression when tumor diameter over 1cm，multi-tumors，lymph node metastasis and Ⅲ-Ⅳ AJCC stage（P<0.05）.L1CAM protein and mRNA was expressed in BHT101 and B-CPAP thyroid cancer cell-lines，and highest in BHT101 cells （P<0.05）.L1CAM was not expressen in thyroid NTHY-ORI 3-1 cells.Conclusion:L1CAM is up-regulated in thyroid papillary tissue and cells，and associated with malignant clinical pathological characteristics of thyroid cancer development and metastasis. |
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| Keywords: | thyroid cancer L1CAM gene BHT101 |
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