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| 瘦素对人成骨样细胞MG63 Ⅰ型胶原A1基因表达的影响 | |
| 引用本文： | 彭绵,陈澍,蔡晓华,彭晓容,彭逸潮.瘦素对人成骨样细胞MG63 Ⅰ型胶原A1基因表达的影响[J].中国骨质疏松杂志,2009,15(10). |
| 作者姓名： | 彭绵 陈澍 蔡晓华 彭晓容 彭逸潮 |
| 作者单位： | 1. 汕头,汕头大学医学院第一附属医院,广东,515041 2. 广州医学院第二附属医院 3. 广东省普宁市人民医院 |
| 基金项目： | 广东省卫生厅科研课题 |
| 摘 要： | 目的 探讨瘦素对人成骨样细胞MG63 Ⅰ型胶原A1基因表达的影响.方法 MG63细胞以3个不同浓度的瘦素(10~(-8)～10~(-6) mol/L)分别干预24、48、72 h,用实时荧光定量PCR法检测MG63细胞Ⅰ型胶原A1基因 mRNA的表达量,分析量效关系和时效关系,以17β-雌二醇作为阳性对照.结果 瘦素可上调MG63细胞Ⅰ型胶原A1基因 mRNA的表达,并存在浓度依赖和时间依赖效应,其最佳浓度为10~(-7) mol/L,最佳作用时间点为72 h.17β-雌二醇则以10~(-7) mol/L浓度组在24 h表达为最强.结论 瘦素可上调MG63细胞Ⅰ型胶原A1基因mRNA的表达,其作用较17β-雌二醇持久和滞后. |
| 关 键 词： | 瘦素 实时荧光定量PCR Ⅰ型胶原 Alpha1 (Ⅰ) collagen gene |
Effects of leptin on expression of alpha1 (Ⅰ) collagen gene in human osteoblast-like cell line MG63 cells |
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| Abstract: | Objective To examine the effects of leptin on alpha1 (Ⅰ) collagen gene expression in human osteoblast-like cell line MG63. Methods MG63 cells were incubated with different doses of leptin (10~(-8)、10~(-7)、10~(-6) mol/L) for 24、48、72 h. The real time flourescence quantitive PCR (FQ-PCR) was performed to detect the mRNA expression of alpha1 (Ⅰ) collagen gene in MG63 cells, with 17β-E_2 as positive control. Results Alpha1 (Ⅰ) collagen gene expression regulated by leptin showed a dose-dependent and time-dependent manners, with the best effect concentration at 10~(-7) mol/L, and maximal expression at 72 h. As positive control, 17β-E_2 also reached its maximal effect in 10~(-7) mol/L group at 24 h. Conclusions Leptin up-regulated alpha1 (I) collagen gene expression in MG63, which is more enduring and later than that of 17β-E_2. |
| Keywords: | MG63 Leptin MG63 Real time flourescence quantitive PCR |
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