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| 成纤维细胞激活蛋白在大鼠烫伤创面修复过程中的动态表达 | |
| 引用本文： | 井刚,陈静,王甲汉.成纤维细胞激活蛋白在大鼠烫伤创面修复过程中的动态表达[J].南方医科大学学报,2013,33(4):615-617. |
| 作者姓名： | 井刚 陈静 王甲汉 |
| 作者单位： | 南方医科大学南方医院烧伤科,广东广州,510515 |
| 摘 要： | 目的观测成纤维细胞激活蛋白（FAP）在大鼠不同深度烫伤创面修复过程中的动态表达特点。方法制作大鼠浅Ⅱ°和深 Ⅱ°两组烫伤模型（每组42只），每组在伤后6、12 h，1、3、7、14、21 d 各处死6只大鼠，另设6只正常大鼠皮肤作为对照。免疫组织 化学和免疫印迹技术观测创面组织FAP的含量。结果FAP在创面成纤维细胞的细胞膜和细胞质表达，尤其是创面新生血管周 围。烫伤后6 h FAP表达明显增多。浅Ⅱ°创面6 h与12 h，1 d与3 d比较，FAP的表达差异无统计学意义（P>0.05），余各时间点 与相邻时间点比较差异均有统计学意义（P<0.05）。深Ⅱ°创面12 h 与1 d，1 d 和3 d 比较，FAP的表达差异无统计学意义（P> 0.05），余各时间点与相邻时间点比较差异均有统计学意义（P<0.05）。两组创面FAP表达的高峰均出现在烫伤后第7天，之后逐 渐下降，至伤后第21天FAP的表达量仍高于对照组，差异有统计学意义（P<0.05）。结论FAP的动态表达特点提示它在创面修 复过程中起重要作用。 |
| 关 键 词： | 成纤维细胞激活蛋白 烫伤创面 修复 表达 |
Expressions of fibroblast activation protein during skin scald burn healing in rats |
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| JING Gang , CHEN Jing , WANG Jiahan.Expressions of fibroblast activation protein during skin scald burn healing in rats[J].Journal of Southern Medical University,2013,33(4):615-617. | |
| Authors: | JING Gang CHEN Jing WANG Jiahan |
| Affiliation: | Department of Burns,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China |
| Abstract: | Objective To investigate the time course of changes in the expression of fibroblast activation protein (FAP) during healing of skin scald burns in rats. Methods Adult Wistar rats were randomized into two equal groups (n=42) and subject to superficial second degree and deep second degree scald burns on the dorsal skin groups, with 6 normal rats serving as the control group. At 6 h, 12 h, and 1, 3, 7, 14, and 21days after burns, 6 rats from each group were sacrificed to detect FAP expression by immunohistochemistry and Western blotting. Results FAP was expressed on the cell membrane and in the cytoplasm of the fibroblasts, especially those around the neovessels. In both burn groups, FAP expression increased significantly at 6 h after burns. In superficial burn group, FAP expression was comparable between 6 and 12 h and between 1 and 3 days (P>0.05), but showed significant differences between the other time points (P<0.05). In deep burn group, FAP expression was comparable between 12 h, 1 day and 3 days (P>0.05) but differed significantly between the other time points (P< 0.05). In both burn groups, FAP expression reached the peak level at 7 days followed by a gradual declination. At 21 days after the burns, FAP maintained a significantly higher expression level than the control level (P<0.05). Conclusion The time course of the changes of FAP expression following scald burns suggests an important role of FAP in the healing process of scald burns. |
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