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Comparison of Two PCRs for Detection of Mycobacterium ulcerans |
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| Authors: | A. Guimaraes-Peres F. Portaels P. de Rijk K. Fissette S. R. Pattyn J.-P. van Vooren P.-A. Fonteyne |
| Abstract: | Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive. |
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