| 白藜芦醇抑制MCF-7乳腺癌细胞增殖的机制研究 |
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| 引用本文： | 郭慧琳,张献全.白藜芦醇抑制MCF-7乳腺癌细胞增殖的机制研究[J].中国肿瘤临床,2011,38(23):1424-1426. |
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| 作者姓名： | 郭慧琳 张献全 |
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| 作者单位： | 重庆医科大学附属第二医院肿瘤科 (重庆市400010) |
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| 摘 要： | 研究白藜芦醇对MCF-7乳腺癌细胞抑制效应及其作用机制。方法:以人MCF-7乳腺癌细胞株为研究对象，利用MTT方法研究白藜芦醇抑制MCF-7乳腺癌细胞的生物学效应；观察在ERK1/2抑制剂PD98059预处理情况下，白藜芦醇抑制MCF-7乳腺癌细胞增殖效应的改变；利用免疫印迹方法观察白藜芦醇对MCF-7乳腺癌细胞中ERK1/2与AKT信号分子的蛋白表达。结果:白藜芦醇能够明显降低MCF-7乳腺癌细胞增殖能力，该作用呈一定的浓度依赖性关系。在ERK1/2抑制剂PD98059预处理情况下，白藜芦醇对MCF-7乳腺癌细胞增殖抑制效应能明显抑制，PD98059可明显减轻该效应。同时，白藜芦醇明显增加p-ERK1/2蛋白表达，降低p-AKT表达水平，但对ERK1/2与AKT蛋白表达无改变。结论:白藜芦醇能够有效抑制MCF-7乳腺癌细胞增殖，该效应与白藜芦醇对ERK1/2及AKT信号途径的调节有关。
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| 关 键 词： | 白藜芦醇 MCF-7乳腺癌细胞 增殖 ERK1/2 AKT |
| 收稿时间： | 2011-06-27 |
The Inhibitory Action of Resveratrol on Proliferation of MCF-7 Breast Cancer Cells |
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| Huilin GUO,Xianquan ZHANG.The Inhibitory Action of Resveratrol on Proliferation of MCF-7 Breast Cancer Cells[J].Chinese Journal of Clinical Oncology,2011,38(23):1424-1426. |
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| Authors: | Huilin GUO Xianquan ZHANG |
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| Affiliation: | Department of Oncology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China |
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| Abstract: | Abstract Objective: To investigate the inhibitory action of resveratrol on the proliferation of MCF-7 breast cancer cells and its underlying mechanisms. Methods: Human MCF-7 breast cancer cells were used and stimulated with resveratrol. The proliferation of MCF-7 breast cancer cells was determined using MTT assay. The changes in proliferation rate were also observed in cells co-stimulated with PD98059, an ERK1/2 inhibitor, and resveratrol. The effects of resveratrol expression on ERK1/2, p-ERK1/2, AKT, and p-AKT in the MCF-7 cancer cells were determined using immunoblotting. Results: The proliferation of MCF-7 breast cancer cells was obviously inhibited by resveratrol in a concentration-dependent manner. The inhibitory action of resveratrol on the MCF-7 cells was overtly repressed by PD98059. At the same time, resveratrol apparently increased p-ERK1/2 protein expression and decreased p-AKT protein expression. However, there was no change in the level of ERK1/2 and AKT protein expression after the resveratrol stimulation. Conclusion: Resveratrol effectively inhibits the proliferation of MCF-7 breast cancer cells, and its inhibitory action is through the regulation of the ERK1/2 and AKT signal pathway. |
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