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| 荷IL-12新城疫病毒治疗结肠癌的实验及其机制探讨 | |
| 引用本文： | 王凤娇,韩继武,王大秀. 荷IL-12新城疫病毒治疗结肠癌的实验及其机制探讨[J]. 现代肿瘤医学, 2021, 0(5): 761-765. DOI: 10.3969/j.issn.1672-4992.2021.05.009 |
| 作者姓名： | 王凤娇 韩继武 王大秀 |
| 作者单位： | 哈尔滨医科大学附属第四医院消化内科，黑龙江 哈尔滨 150001 |
| 摘 要： | 目的:探讨荷IL-12新城疫病毒rClone30-IL-12瘤内注射治疗结肠癌的作用效果及可能机制.方法:RT-PCR法鉴定病毒中IL-12;构建荷CT26细胞的结肠癌模型,将荷瘤小鼠随机分为rClone30-IL-12组、奥沙利铂(OXA)组、磷酸盐缓冲液(PBS)组;给药后隔天记录瘤体直径(mm)、小鼠质量(g)和... |
| 关 键 词： | 重组新城疫病毒 结肠癌 白介素-12 NK细胞 |
The experiment and mechanism study of Newcastle disease virus containing IL-12 in treating colon cancer |
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| WANG Fengjiao,HAN Jiwu,WANG Daxiu. The experiment and mechanism study of Newcastle disease virus containing IL-12 in treating colon cancer[J]. Journal of Modern Oncology, 2021, 0(5): 761-765. DOI: 10.3969/j.issn.1672-4992.2021.05.009 | |
| Authors: | WANG Fengjiao HAN Jiwu WANG Daxiu |
| Affiliation: | Department of Gastoenterology，the Fourth Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin 150001,China. |
| Abstract: | Objective:To investigate the effect and possible mechanism of rClone30-IL-12 in the treatment of colon cancer by intratumoral injection.Methods:Identifying IL-12 in viral rClone30-IL-12 by RT-PCR.Constructing the colon cancer model,the tumor-bearing mice were randomly divided into rClone30-IL-12 group,oxaliplatin(OXA) group and phosphate buffer solution(PBS) group.Recording the diameter of the tumor(mm),the weight of the mouse(g) and the state of daily life every other day after administration.Dissecte the spleen,tumor and recorde the tumor weight.Detect the expression of NK cells in spleen of tumor-bearing mice by flow cytometry.Results:rClone30-IL-12 virus contained IL-12 gene.The average tumor volume and tumor weight of the tumor-bearing mice in the rClone30-IL-12 group and the OXA group were significantly lower than those in the PBS group(P<0.01,P<0.01,P<0.01,P<0.05).The average body weight of tumor-bearing mice in the OXA group was significantly lower than that in the rClone30-IL-12 group and the PBS group(P<0.01,P<0.01).The proportion of NK cells in the spleen of rClone30-IL-12 group tumor-bearing mice was significantly higher than that of PBS group and OXA group.Conclusion:Intratumoral injection of rClone30-IL-12 can effectively inhibit the growth of colon cancer cells,and its mechanism may be related to the oncolytic effect of Newcastle disease virus and the activation of NK cells stimulated by rClone30-IL-12 release of IL-12.Compared with OXA,they has similar clinical effects,but rClone30-IL-12 has less toxic side effects. |
| Keywords: | recombinant Newcastle disease virus colon cancer interleukin-12 NK cells |
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