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| Claudin-1蛋白在胃癌组织和细胞中的表达及促进其表达后对癌细胞增殖、侵袭和迁移的影响 | |
| 引用本文： | 郭晓光,唐治蓉,韩莹,廖文华,张涛. Claudin-1蛋白在胃癌组织和细胞中的表达及促进其表达后对癌细胞增殖、侵袭和迁移的影响[J]. 现代肿瘤医学, 2021, 0(6): 934-940 |
| 作者姓名： | 郭晓光 唐治蓉 韩莹 廖文华 张涛 |
| 作者单位： | 南充市中心医院临床病理科 |
| 基金项目： | 四川省科技厅面上项目(编号:2018JY0417);南充市科学技术和知识产权局省级科技计划项目(编号:2018JY0416)。 |
| 摘 要： | 目的:研究紧密连接跨膜蛋白(Claudin-1)在胃癌组织中的表达及其对胃癌细胞生物学行为及裸鼠皮下成瘤能力的影响.方法:收集50例胃癌患者的癌组织及其癌旁组织,采用RT-qPCR和免疫组化检测Claudin-1的表达;RT-qPCR和Western Blot检测Claudin-1在人胃癌细胞株MKN45、SGC790... |
| 关 键 词： | Claudin-1 胃癌 增殖 侵袭 迁移 EMT |
Expression of Claudin-1 protein in gastric cancer tissues and cells and its effect on cancer cell proliferation,invasion and migration after promoting its expression |
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| GUO Xiaoguang,TANG Zhirong,HAN Ying,LIAO Wenhua,ZHANG Tao. Expression of Claudin-1 protein in gastric cancer tissues and cells and its effect on cancer cell proliferation,invasion and migration after promoting its expression[J]. Journal of Modern Oncology, 2021, 0(6): 934-940 | |
| Authors: | GUO Xiaoguang TANG Zhirong HAN Ying LIAO Wenhua ZHANG Tao |
| Affiliation: | (Department of Clinicopathology,Nanchong Central Hospital,Sichuan Nanchong 637000,China) |
| Abstract: | Objective:To study the expression of tight junction transmembrane protein(Claudin-1)in gastric cancer tissues and its effect on the biological behavior of gastric cancer cells and the ability of subcutaneous tumor formation in nude mice.Methods:The cancer tissues and adjacent tissues of 50 patients with gastric cancer were collected,and the expression level of Claudin-1 in tissues was detected by RT-qPCR and immunohistochemistry.RT-qPCR and Western Blot were used to detect the expression level of Claudin-1 in human gastric cancer cell line MKN45,human gastric carcinoma SGC7901 cell line,human gastric carcinoma MKN28 cell line and normal human immortalized gastric epithelial cells(GES-1).Claudin-1 was overexpressed in human gastric cancer cell line SGC7901 by lentivirus.The cells were divided into control group(untransfected),NC group(transfected with lentiviral vector)and Claudin-1 group(transfected with Claudin-1 overexpressing lentivirus).Cell Counting Kit-8(CCK-8),Transwell test,and scratch test were used to detect changes in gastric cancer cell proliferation,invasion,and migration ability.Western Blot and immunofluorescence experiments were used to detect the expression of Claudin-1 on epithelial-mesenchymal transition(EMT)marker proteins E-cadherin and N-cadherin.Ten 4-week-old male BALB/c nude mice were injected subcutaneously with Claudin-1 overexpressing lentivirus-treated SGC7901 cells to construct a xenograft model,and the effect of Claudin-1 overexpression on subcutaneous xenografts was studied.Kaplan-Meier website was used to analysis the relationship between Claudin-1 expression and patient survival.Results:Immunohistochemical results showed that the expression level of Claudin-1 in gastric cancer tissue was significantly lower than that in adjacent tissues(P<0.05).Overexpression of Claudin-1 could significantly inhibit the proliferation,invasion and migration ability of SGC7901 cells(P<0.05).At the same time,the expression of E-cadherin was increased and the expression of N-cadherin was decreased.Overexpression of Claudin-1 in a nude mouse xenograft model can significantly inhibit the growth of subcutaneous xenografts(P<0.05).Patients with high expression of Claudin-1 survive longer than patients with low expression of Claudin-1.Conclusion:Claudin-1 is underexpressed in gastric cancer tissues.Overexpression of Claudin-1 inhibits the proliferation,invasion,migration,and EMT of gastric cancer cells and inhibits the ability of subcutaneous tumor formation in nude mice.Claudin-1 high expression promotes good prognosis for patients. |
| Keywords: | Claudin-1 gastric cancer proliferation invasion migration EMT |
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