| 叶酸缺乏导致小鼠听力障碍机制的实验研究 |
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| 引用本文： | 蔡润茁,马云胜,左中夫.叶酸缺乏导致小鼠听力障碍机制的实验研究[J].中国耳鼻咽喉头颈外科,2017,24(12):620-624. |
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| 作者姓名： | 蔡润茁 马云胜 左中夫 |
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| 基金项目： | 辽宁省自然科学基金(20170540394)，锦州医科大学奥鸿博泽基金(173914612) |
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| 摘 要： | 目的 阐明叶酸缺乏后小鼠听力损失的影响因素和机制，为临床诊疗提供依据。方法 两组小鼠分别以低叶酸饮食和正常叶酸饮食喂养10周。ABR听力测试。处死动物，心脏抽血检测血清同型半胱氨酸和叶酸量。观察两组小鼠耳蜗形态学变化。免疫印记法分析相关蛋白表达情况。TUNEL法检测小鼠耳蜗细胞凋亡情况。结果 分析显示叶酸缺乏组小鼠血清叶酸水平下降，同型半胱氨酸水平升高。ABR检查和耳蜗细胞凋亡数量发现叶酸缺乏组小鼠听力损失严重。免疫印迹检测揭示叶酸缺乏组小鼠比正常组耳蜗同型半胱氨酸升高50%，提示小鼠耳蜗高同型半胱氨酸血症。结论 高同型半胱氨酸血症和叶酸缺乏引发小鼠听力损失，与内耳同型半胱氨酸代谢障碍有关。
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| 关 键 词： | 叶酸缺乏 小鼠 听力障碍 动物实验 |
Experimental research of mechanism of hearing impairment cause by lack of folic acid in mouse |
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| Abstract: | OBJECTIVE To clarify the influence factors of hearing impairment in mice caused by lack of folic acid and the mechanism.METHODS Mice were randomly divided into 2 groups,fed with no folic acid diet and normal diet for 10 weeks.Hearing was tested by ABR.Serum homocysteine,folic acid were detected.Immune imprint method was used to analyze the expression of related protein.Cochlear tissue was tested by histological method to observe morphological changes.At the same time using the TUNEL method was used to analyse the cochlear tissue cell apoptosis.RESULTS Analysis showed that serum folate levels in mice fed without folic acid diet dropped than that of the normal group,while homocysteine levels elevated.Through the brain stem auditory records and cochlear cell apoptosis of mice severe hearing loss was found in folate deficiency group.Western blot detection showed enzyme catalysis homocysteine products increased by 50％ in folate deficiency group than that of the normal diet group.CONCLUSION The high homocysteine levels,and folate deficiency can cause hearing loss in mice and are related with the inner ear homocysteine metabolic disorders. |
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