| 高效液相色谱测定大鼠血浆儿茶酚胺的含量 |
| |
| 引用本文： | 裴兆辉,朱妙章,裴建明,高瞻,吕顺艳. 高效液相色谱测定大鼠血浆儿茶酚胺的含量[J]. 心脏杂志, 2003, 15(1): 9-11. DOI: 10.13191/j.chj.2003.01.11.peizhh.004 |
| |
| 作者姓名： | 裴兆辉 朱妙章 裴建明 高瞻 吕顺艳 |
| |
| 作者单位： | 第四军医大学生理学教研室,陕西,西安,710032 |
| |
| 摘 要： | 目的 :改进高效液相色谱配电化学检测器检测血浆儿茶酚胺 （CA）类物质含量的技术。方法 :用不同的流动相条件 ,在体外用外标法对大鼠血浆 CA含量进行检测。结果 :色谱条件以流动相用双蒸水配制 ,脱气 3 0 min,p H 2 .8～ 3 .0 ,检测电压 0 .6V,流速 0 .6m l/ min外标法为理想检测条件。去甲肾上腺素 （NE）最小检测极限 1.0 0 3 ng/ ml,肾上腺素 （E）最小检测极限 1.2 1ng/ ml。结论 :本文方法简便、快速、可靠 ,可用于科研测定血浆 NE和 E
|
| 关 键 词： | 大鼠 儿茶酚胺 高效液相色谱 电化学检测器 |
| 文章编号： | 1009-7236(2003)01-0009-03 |
To detect the contents of mice plasma catecholamine by the high efficient liquid chromatography |
| |
| PEI Zhao hui,ZHU Miao zhang,PEI Jian ming,GAO Zhan,L Shun yan. To detect the contents of mice plasma catecholamine by the high efficient liquid chromatography[J]. Chinese Heart Journal, 2003, 15(1): 9-11. DOI: 10.13191/j.chj.2003.01.11.peizhh.004 |
| |
| Authors: | PEI Zhao hui ZHU Miao zhang PEI Jian ming GAO Zhan L Shun yan |
| |
| Abstract: | AIM:To improve the technique of the high efficient liquid chromatography of mice plasma catecholamine to study the changes of plasma catecholammine (CA) content. METHODS:To measure the plasma CA content of bound stress mice labeled in vitro by the method of high efficient liquid chromatography electrochemical detector. RESULTS:Chromatography conditions were made with the flow phas double steam water and degassed completely,pH 2.8~3.0,detecting voltage 0.6 V,flow speed 0.6 ml/min. The detected smallest limit of norepinephrine(NE) is 1.003 ng/ml,the detected smallest limit of epinephrine(E) is 1.21 ng/ml. This way is very idea.CONCLUSION:This measure method is simple, swift and confident;it can be used to detect the plasmas NE and E in clinic and basic research. |
| |
| Keywords: | rat catecholammine high efficient liquid chromatography electrochemical detector |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《心脏杂志》浏览原始摘要信息 |
|
点击此处可从《心脏杂志》下载免费的PDF全文 |
|
