| circ_0001721/miR-198对前列腺癌细胞生物学行为的影响 |
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| 引用本文： | 王红梅,谢 斌,陈寒超,关 虹,李春芸.circ_0001721/miR-198对前列腺癌细胞生物学行为的影响[J].现代肿瘤医学,2023,0(14):2575-2580. |
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| 作者姓名： | 王红梅 谢 斌 陈寒超 关 虹 李春芸 |
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| 作者单位： | 1.中南大学湘雅医学院附属海口医院检验科；2.重症医学科，海南 海口 570203 |
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| 基金项目： | 海南省卫生计生行业科研项目（编号:19A200164） |
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| 摘 要： | 目的:探讨circ_0001721/miR-198分子轴对人前列腺癌细胞DU145生物学行为的影响及相关作用机制。方法:应用qRT-PCR法检测circ_0001721、miR-198的表达量；DU145细胞转染si-circ_0001721、miR-198 mimics、anti-miR-198以及相应的对照（si-NC、miR-NC、anti-miR-NC）；CCK-8法、平板克隆形成实验、流式细胞术、Transwell实验检测细胞增殖、克隆、凋亡、迁移及侵袭；双荧光素酶报告实验验证circ_0001721与miR-198的靶向关系；Western blot检测E-cadherin、N-cadherin蛋白表达量。结果:与癌旁组织比较，circ_0001721在前列腺癌组织中高表达（P＜0.05），而miR-198在前列腺癌组织中低表达（P＜0.05）；与转染si-NC或转染miR-NC相比，si-circ_0001721或miR-198 mimics可以提高细胞增殖抑制率、凋亡率和E-cadherin蛋白水平（P＜0.05），降低克隆形成数、迁移和侵袭数以及N-cadherin蛋白水平（P＜0.05）；circ_0001721可靶向结合miR-198；与共转染si-circ_0001721和anti-miR-NC相比，共转染si-circ_0001721和anti-miR-198降低了细胞增殖抑制率、凋亡率和E-cadherin蛋白水平（P＜0.05），而增加了克隆形成数、迁移和侵袭数以及N-cadherin蛋白水平（P＜0.05）。结论:敲低circ_0001721可通过上调miR-198抑制前列腺癌细胞的生长和转移。
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| 关 键 词： | 前列腺癌 circ_0001721 miR-198 增殖 迁移 侵袭 凋亡 |
The effect of circ_0001721/miR-198 on the biological behavior of prostate cancer cells |
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| WANG Hongmei,XIE Bin,CHEN Hanchao,GUAN Hong,LI Chunyun.The effect of circ_0001721/miR-198 on the biological behavior of prostate cancer cells[J].Journal of Modern Oncology,2023,0(14):2575-2580. |
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| Authors: | WANG Hongmei XIE Bin CHEN Hanchao GUAN Hong LI Chunyun |
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| Affiliation: | 1.Department of Laboratory Medicine;2.Department of Critical Care Medicine,Haikou Affiliated Hospital of Central South University Xiangya School of Medicine,Hainan Haikou 570203,China. |
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| Abstract: | Objective:To determine the impact of circ_0001721/miR-198 molecular axis on the biological behavior of human prostate cancer cells DU145 and its related mechanism.Methods:The expression levels of circ_0001721 and miR-198 were detected by qRT-PCR.DU145 cells were transfected with si-circ_0001721,miR-198 mimics,anti-miR-198 and their negative controls (si-NC,miR-NC,anti-miR-NC),respectively.Cell proliferation,cloning,apoptosis,migration and invasion were assessed using CCK-8,colony formation assay,flow cytometry and Transwell assay,respectively.Targeting between circ_0001721 and miR-198 was validated using dual-luciferase reporter assay.The protein expression of E-cadherin and N-cadherin was detected by Western blot.Results:Compared with normal tissue,circ_0001721 expression in prostate cancer tissue was increased (P＜0.05),while miR-198 expression in prostate cancer tissue was decreased (P＜0.05).Compared with transfection of si-NC or miR-NC,the cell proliferation inhibition rate,apoptosis rate and the protein level of E-cadherin were increased (P＜0.05),while the number of cell clone formation,migration,invasion and the protein level of N-cadherin were decreased (P＜0.05) after transfection of si-circ_0001721 or miR-198 mimics.circ_0001721 could target miR-198.Compared with co-transfection with si-circ_0001721 and anti-miR-NC,the cell proliferation inhibition rate,apoptosis rate and the protein level of E-cadherin were reduced (P＜0.05),while the number of cell clone formation,migration,invasion and the protein level of N-cadherin were increased (P＜0.05) after co-transfection with si-circ_0001721 and anti-miR-198.Conclusion:Inhibition of circ_0001721 expression could reduce prostate cancer cell growth and metastasis by up-regulating miR-198. |
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| Keywords: | prostate cancer circ_0001721 miR-198 proliferation migration invasion apoptosis |
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