| 新多胺类似物TBP对人膀胱移行细胞癌T24细胞凋亡及迁移能力的影响 |
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| 引用本文： | 杨建林,韩钰,王凯,张贺吉,王艳林.新多胺类似物TBP对人膀胱移行细胞癌T24细胞凋亡及迁移能力的影响[J].中国肿瘤临床,2012,39(23):1869-1872. |
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| 作者姓名： | 杨建林 韩钰 王凯 张贺吉 王艳林 |
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| 作者单位： | ①.三峡大学医学院分子生物学研究所（湖北省宜昌市443002） |
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| 基金项目： | 国家自然科学基金项目(编号:30772590)资助~~ |
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| 摘 要： | 目的 研究新多胺类似物四丁基丙二胺(tetrabutyl propaneadiamine, TBP)对人膀胱移行细胞癌T24细胞生长、凋亡及迁移能力的影响。 方法 MTT比色法分析细胞增殖, 流式细胞术分析细胞周期改变, TUNEL法检测细胞凋亡, Western blot用于分析细胞凋亡相关蛋白Bax和细胞色素C的表达水平, Transwell技术检测细胞迁移能力的变化。 结果 TBP有效抑制T24细胞增殖及迁移能力。细胞周期分析显示TBP抑制细胞发生G_1/S期转换, 导致S期细胞比例显著性下降, G_0/G_1期细胞比例升高, 同时出现SubG_1亚凋亡峰。TBP处理后, 细胞呈现典型凋亡现象, 细胞浆中促凋亡蛋白Bax和细胞色素C含量明显升高。 结论 TBP能够抑制人膀胱移行细胞癌T24细胞增殖、降低T24细胞迁移能力, 诱导细胞凋亡, 其机制可能与影响肿瘤细胞的细胞周期, 活化线粒体相关的细胞凋亡途径有关。
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| 关 键 词： | 多胺类似物 四丁基丙二胺 膀胱癌细胞 迁移 凋亡 |
| 收稿时间： | 2012-06-20 |
Effects of the New Polyamine Analog Tetrabutyl Propanediamine on the Apoptosis and Migration of Human Bladder Carcinoma T24 Cells |
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| Jianlin YANG,Yu HAN,Kai WANG,Heji ZHANG,Yanlin WANG.Effects of the New Polyamine Analog Tetrabutyl Propanediamine on the Apoptosis and Migration of Human Bladder Carcinoma T24 Cells[J].Chinese Journal of Clinical Oncology,2012,39(23):1869-1872. |
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| Authors: | Jianlin YANG Yu HAN Kai WANG Heji ZHANG Yanlin WANG |
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| Affiliation: | ①.Institute of Molecular Biology, Medical College of Three Gorges University, Yichang 443002, China②.Wuhan Institute of Technology, Hubei Key Laboratory of Novel Reactor & Green Chemical Technology,Wuhan 430073, China |
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| Abstract: | Objective This work aimed to investigate the effects of the new polyamine analog tetra-butyl propanediamine (TBP) on the proliferation, apoptosis, and migration of human bladder carcinoma T24 cells and its potential mechanism. Methods Methyl thiazolyl tetrazolium was used to analyze cell proliferation, and flow cytometry was performed to assay the cell cycles. Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling assay was used to identify the apoptotic cells. Western blot analysis was used to evaluate the expression of apoptosis-related proteins. The Transwell technique was used to analyze cell migration. Results TBP significantly inhibited the proliferation and migration of T24 cells, and the inhibitory effect was time and dose dependent. The flow cytometry results indicated that TBP interfered with the cell cycles and inhibited G1/S transition, which led to increased cell percentage in the G1 and G2 phases, as well as decreased cell percentage in the S phase. Meanwhile, the number of apoptotic cells significantly increased. After TBP treatment, observation of the typical behavior of apoptotic cells revealed that the contents of the pro-apoptotic proteins Bax and cytochrome C significantly increased in the cytosol. Conclusion TBP can inhibit the proliferation and induce the apoptosis of human bladder carcinoma T24 cells by interfering with the cell cycle and activating the mitochondrion-mediated apoptotic pathway. |
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