| 莫诺苷对急性心肌梗死大鼠梗死心肌周边组织中Ang-1及FGF-2表达的影响 |
| |
| 引用本文： | 王天博,崔加敏,刘婷婷,孙芳玲,陶依然,吴铮,王文,夏云峰.莫诺苷对急性心肌梗死大鼠梗死心肌周边组织中Ang-1及FGF-2表达的影响[J].心脏杂志,2019,31(3):266-269. |
| |
| 作者姓名： | 王天博 崔加敏 刘婷婷 孙芳玲 陶依然 吴铮 王文 夏云峰 |
| |
| 作者单位： | 1.解放军总医院第一附属医院干部一科，北京 100048 |
| |
| 摘 要： | 目的 观察莫诺苷对急性心肌梗死大鼠梗死心肌周边组织中血管生成素(Ang)-1及成纤维细胞生长因子(FGF)-2表达的影响。方法 选取体质量(260~280)g的雄性SD大鼠20只,采用结扎冠状动脉左前降支的方法制备AMI大鼠模型。造模成功后的大鼠随机分为假手术组、模型组、莫诺苷低剂量组(45 mg/kg)、莫诺苷中剂量组(90 mg/kg)和莫诺苷高剂量组(180 mg/kg)。从造模后第1天开始,采用灌胃方式每天定时给药,假手术组和模型组给予等量的蒸馏水。造模大鼠在连续给药7 d后处死取材,Western blot检测梗死心肌周边组织中Ang-1及FGF-2的蛋白表达量。结果 与假手术组相比,模型组Ang-1的蛋白表达量明显升高(P<0.01),而FGF-2表达量有升高趋势,但无显著性差异;与模型组相比,莫诺苷高剂量组Ang-1、FGF-2蛋白表达量显著提高(P<0.01,P<0.05)。结论 莫诺苷能上调急性心肌梗死大鼠梗死心肌周边组织中Ang-1及FGF-2表达,促进血管新生。
|
| 关 键 词： | 莫诺苷 急性心肌梗死 血管生成素1 成纤维细胞生长因子2 血管新生 |
| 收稿时间： | 2019-02-18 |
Effects of morroniside on expression of angiopoietin-1 and fibroblast growth factor-2 in infarcted myocardium in rats with acute myocardial infarction |
| |
| WANG Tian-bo,CUI Jia-min,LIU Ting-ting,SUN Fang-ling,TAO Yi-ran,WU Zheng,WANG Wen,XIA Yun-feng.Effects of morroniside on expression of angiopoietin-1 and fibroblast growth factor-2 in infarcted myocardium in rats with acute myocardial infarction[J].Chinese Heart Journal,2019,31(3):266-269. |
| |
| Authors: | WANG Tian-bo CUI Jia-min LIU Ting-ting SUN Fang-ling TAO Yi-ran WU Zheng WANG Wen XIA Yun-feng |
| |
| Affiliation: | 1.First Department of Cadres’ Ward, First Affiliated Hospital, PLA General Hospital, Beijing 100048, China2.Experimental Animal Center, Xuanwu Hospital of Capital Medical University, Beijing 100053, China |
| |
| Abstract: | AIM To observe the effects of morroniside on the expressions of angiopoietin 1 (Ang-1) and fibroblast growth factor 2 (FGF-2) in the infarcted myocardium of rats with acute myocardial infarction. METHODS Twenty male Sprague-Dawley rats weighing 260-280g were selected and an AMI rat model was prepared by ligation of the left anterior descending coronary artery. The rats after successful modeling were randomly divided into a sham operation group, a model group, a low dose group of morroniside (45 mg/kg), a middle dose group of morroniside (90 mg/kg), and a high dose group of morroniside (180 mg/kg). From the first day after modeling, the rats were given morroniside regularly by gavage, and the sham operation and model groups were given the same amount of distilled water. The model rats were sacrificed 7 days after continuous administration and the protein expression levels of Ang-1 and FGF-2 in the infarcted myocardium were detected by Western blot. RESULTS Compared with those in the sham operation group, the protein expression of Ang-1 in the model group was significantly increased (P < 0.01), while the expression of FGF-2 showed an increasing tendency, but there was no significant difference (P>0.05). Compared with those in the model group, the expression levels of both Ang-1 and FGF-2 in the high dose group of morroniside were significantly increased (P < 0.01, P < 0.05). CONCLUSION Moroniside can up-regulate the expression of Ang-1 and FGF-2 in the infarcted myocardium of rats with acute myocardial infarction and promote angiogenesis. |
| |
| Keywords: | Monoside acute myocardial infarction angiopoietin-1 fibroblast growth factor-2 angiogenesis |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《心脏杂志》浏览原始摘要信息 |
|
点击此处可从《心脏杂志》下载全文 |
|
