| PIM1基因通过调控STAT3信号通路蛋白表达对食管癌细胞生物学行为的影响 |
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| 引用本文： | 徐汉桥,潘捷,田学涛,陈志强. PIM1基因通过调控STAT3信号通路蛋白表达对食管癌细胞生物学行为的影响[J]. 现代肿瘤医学, 2021, 0(3): 392-396. DOI: 10.3969/j.issn.1672-4992.2021.03.006 |
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| 作者姓名： | 徐汉桥 潘捷 田学涛 陈志强 |
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| 作者单位： | 江汉大学附属医院 武汉市六医院胸外科，湖北 武汉 430015 |
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| 摘 要： | 目的:探讨原癌基因PIM1通过调控信号转导子与激活子3(STAT3)信号通路对食管癌KYSE150细胞增殖、凋亡、侵袭和迁移的影响.方法:分别以PIM1 siRNA和siRNA Control转染KYSE150细胞,命名为PIM1-siRNA组和NC组,同时设置空白对照Control组.采用实时荧光定量PCR(qRT-...
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| 关 键 词： | PIM1基因 STAT3信号通路 增殖 凋亡 侵袭 迁移 |
Effect of PIM1 gene on proliferation,apoptosis,invasion and migration of esophageal cancer cells through regulating STAT3 signaling pathway protein expression |
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| XU Hanqiao,PAN Jie,TIAN Xuetao,CHEN Zhiqiang. Effect of PIM1 gene on proliferation,apoptosis,invasion and migration of esophageal cancer cells through regulating STAT3 signaling pathway protein expression[J]. Journal of Modern Oncology, 2021, 0(3): 392-396. DOI: 10.3969/j.issn.1672-4992.2021.03.006 |
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| Authors: | XU Hanqiao PAN Jie TIAN Xuetao CHEN Zhiqiang |
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| Affiliation: | Department of Thoracic Surgery,Wuhan No.6 Hospital,Jianghan University Affiliated Hospital,Hubei Wuhan 430015,China. |
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| Abstract: | Objective:To investigate the effect of proto-oncogene PIM1 gene on proliferation,apoptosis,invasion and migration of esophageal cancer KYSE150 cells by regulating signal transducer and activator 3(STAT3)signaling pathway.Methods:KYSE150 cells were transfected with PIM1 siRNA and siRNA Control,respectively named PIM1-siRNA group and NC group,and a blank control group was set.Real-time quantitative PCR(qRT-PCR)and Western blot were used to detect the expression of PIM1 in each group.Cell proliferation,apoptosis,invasion and migration were measured by MTT assay,flow cytometry and Transwell assay,respectively.Western blot was used to detect protein expression levels related to cell proliferation,apoptosis,invasion and migration,and STAT3 signaling pathway.Results:The expression of PIM1 mRNA and protein in KYSE150 cells of PIM1-siRNA group was significantly lower than that of Control group(P<0.05).The OD value,invasion and migration number of KYSE150 cells in PIM1-siRNA group were significantly lower than those in Control group(P<0.05),and the apoptosis rate was significantly higher than that in Control group(P<0.05).PCNA,MMP-2,MMP-9 and the expression level of p-STAT3 protein was lower than that of Control group(P<0.05),and the expression level of Cleaved Caspase-3 protein was higher than that of Control group.There was no significant difference between the Control group and the NC group(P>0.05).Conclusion:PIM1 regulates proliferation,apoptosis,invasion and migration of esophageal cancer KYSE150 cells by regulating STAT3 signaling pathway. |
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| Keywords: | PIM1 gene STAT3 signaling pathway proliferation apoptosis invasion migration |
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