miR-593通过调控PLK1基因的表达抑制结肠癌细胞的增殖 |
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引用本文: | 马金珠,朱益平,王箴,昝嘉伟,曹陇,冯遵永,王森林,范倩,颜亮.miR-593通过调控PLK1基因的表达抑制结肠癌细胞的增殖[J].南方医科大学学报,2019,39(2):144. |
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作者姓名: | 马金珠 朱益平 王箴 昝嘉伟 曹陇 冯遵永 王森林 范倩 颜亮 |
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作者单位: | 皖南医学院活性生物大分子安徽省重点实验室,安徽 芜湖,241002;皖南医学院第一附属医院,安徽 芜湖,241022;安徽师范大学生命科学学院,安徽 芜湖,241002;天津市肿瘤医院,天津,300060 |
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基金项目: | 国家自然科学基金;国家自然科学基金;国家自然科学基金;安徽省教育厅自然科学研究重点项目 |
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摘 要: | 目的探讨miR-593在结肠癌细胞增殖中的作用及分子机制。方法利用生物信息学分析筛选miR-593可能结合的靶基
因为PLK1;利用双荧光素酶报告基因实验验证miR-593 和PLK1 基因的结合;通过qRT-PCR、Western blotting 验证PLK1 为
miR-593直接作用的靶基因;通过CCK-8实验证明miR-593通过调控PLK1基因的表达抑制结肠癌细胞的增殖。结果运用三
种生物信息学软件对PLK1可能结合的miRNA进行了预测与分析,发现miR-593可能结合PLK1基因;双荧光素酶报告基因实
验证明miR-593 与PLK1 基因发生了结合;Western blotting 结果表明,PLK1 在miR-593 mimic 转染组表达量显著下降,而在
miR-593 inhibitor 组明显升高,相对于对照组均具有统计学差异(P<0.05);qRT-PCR结果表明,PLK1 RNA水平在各组中表
达水平差异无统计学意义(P>0.05);细胞增殖实验表明,miR-593与PLK1对结肠癌细胞增殖的影响为相反关系,且共转染miR-593
mimic与PLK1过表达质粒组对细胞增殖的影响介于单独转染miR-593 mimic组与PLK1过表达质粒组之间。结论miR-593通
过调控PLK1基因的表达抑制了结肠癌细胞的增殖,并发挥抑癌miRNA的作用。
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关 键 词: | miR-593 结肠癌细胞 PLK1 |
miR-593 inhibits proliferation of colon cancer cells in vitro by down-regulating PLK1 |
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Abstract: | Objective To explore the role of miR-593 in regulating the proliferation of colon cancer cells and the molecular
mechanism. Methods Bioinformatics analysis identified PLK1 as the possible target gene of miR-593. Luciferase assay was
employed to verify the binding between miR-593 and PLK1, and qRT-PCR and Western blotting were used to verify that PLK1
was the direct target gene of miR-593. CCK-8 assay was performed to test the hypothesis that miR-593 inhibited the
proliferation of colon cancer cells by targeting PLK1. Results Luciferase assay identified the specific site of miR-593 binding
with PLK1. Western blotting showed a significantly decreased expression of PLK1 in the colon cancer cells transfected with
miR-593 mimics and an increased PLK1 expression in the cells transfected with the miR-593 inhibitor as compared with the
control cells (P<0.05). The results of qRT-PCR showed no significant differences in the expression levels of PLK1 among the
cells with different treatments (P>0.05). The cell proliferation assay showed opposite effects of miR-593 and PLK1 on the
proliferation of colon cancer cells, and the effect of co-transfection with miR-593 mimic and a PLK1-overexpressing plasmid on
the cell proliferation was between those in PLK1 over-expressing group and miR-593 mimic group. Conclusion miR-593
inhibits the proliferation of colon cancer cells by down-regulating PLK1 and plays the role as a tumor suppressor in colon
cancer. |
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