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| 白藜芦醇上调BTG2表达抑制肝癌HepG2细胞增殖 | |
| 引用本文： | 刘 兰,饶智国,张志敏,付红星. 白藜芦醇上调BTG2表达抑制肝癌HepG2细胞增殖[J]. 现代肿瘤医学, 2019, 0(20): 3556-3560. DOI: 10.3969/j.issn.1672-4992.2019.20.003 |
| 作者姓名： | 刘 兰 饶智国 张志敏 付红星 |
| 作者单位： | 解放军武汉总医院肿瘤内科，湖北 武汉 430070 |
| 基金项目： | National Natural Science Foundation of China(No.81301631）；国家自然科学基金资助项目（编号:81301631）;湖北省卫生计生委科研基金（编号:WJ2018H0067） |
| 摘 要： | 目的:初步探讨白藜芦醇(resveratrol，Res)对肝癌HepG2细胞增殖及BTG2表达的影响。方法:不同浓度Res(终浓度分别为20、40、80 μmol/L)处理HepG2细胞，CCK-8法检测细胞增殖能力，流式细胞术检测各组细胞周期分布变化，RT-PCR、Western blot技术检测BTG2 mRNA和蛋白水平表达的变化。结果:Res可以显著抑制HepG2细胞的增殖，且呈浓度和时间依赖性(P＜0.01)，药物作用48 h后IC50为34.58 μmol/L。Res可使HepG2细胞周期阻滞在G2/M期；随着Res作用浓度的升高，抗增殖基因BTG2 mRNA和蛋白的表达逐渐增加(P＜0.01)。结论:Res能够抑制肝癌HepG2细胞的增殖，对细胞周期具有阻滞效应且有浓度依赖性，其机制可能与其上调抗增殖基因BTG2表达相关。 |
| 关 键 词： | 白藜芦醇 肝癌 细胞增殖 B细胞易位基因2 |
Resveratrol up-regulates the expression of BTG2 and inhibits the proliferation of hepatocellular carcinoma HepG2 cells |
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| Liu Lan,Rao Zhiguo,Zhang Zhimin,Fu Hongxing. Resveratrol up-regulates the expression of BTG2 and inhibits the proliferation of hepatocellular carcinoma HepG2 cells[J]. Journal of Modern Oncology, 2019, 0(20): 3556-3560. DOI: 10.3969/j.issn.1672-4992.2019.20.003 | |
| Authors: | Liu Lan Rao Zhiguo Zhang Zhimin Fu Hongxing |
| Affiliation: | Department of Oncology,Wuhan General Hospital of Chinese People's Liberation Army,Hubei Wuhan 430070,China. |
| Abstract: | Objective:To investigate the effect of resveratrol (Res) on the proliferation and expression of BTG2 in HepG2 cells.Methods:HepG2 cells were treated with different concentrations of Res (20,40,80 μmol/L),CCK-8 assay was used to detect cell proliferation.Flow cytometry was used to detect cell cycle distribution,RT-PCR and Western blot were used to detect the expression of BTG2 mRNA and protein.Results:Res could inhibit the proliferation of HepG2 cells in a concentration and time dependent manner (P<0.01)，and IC50 value was 34.58 μmol/L after 48 hours of drug treatment,Res could block the cell cycle of HepG2 in G2/M phase,with the increase of Res concentration,the expression of BTG2 mRNA and protein were gradually increased (P<0.01).Conclusion:Res can inhibit the proliferation of HepG2 cells in a concentration-dependent manner,and its mechanism may be related to the up-regulation of BTG2 expression. |
| Keywords: | resveratrol hepatocellular carcinoma cell proliferation BTG2 |
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