| Twist1对人乳腺癌细胞E-cadher in表达及亚细胞定位的影响 |
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| 引用本文： | 张冲,孙保存,张丹芳,刘芳,董学易,刘志勇,车娜,赵秀兰. Twist1对人乳腺癌细胞E-cadher in表达及亚细胞定位的影响[J]. 中国肿瘤临床, 2013, 0(9): 509-512. DOI: 10.3969/j.issn.1000-8179.2013.09.005 |
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| 作者姓名： | 张冲 孙保存 张丹芳 刘芳 董学易 刘志勇 车娜 赵秀兰 |
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| 作者单位： | ①.天津医科大学病理教研室（天津市300070） |
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| 基金项目： | 国家自然科学基金重点项目81230050国家自然科学基金青年科学基金项目81101724国家自然科学基金面上项目81172046 |
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| 摘 要： | 目的 探讨转录调控因子Twist1对人乳腺癌MCF-7细胞E-cadherin表达的影响。 方法 上调人乳腺癌MCF-7细胞中Twist1的表达, 利用RT-PCR, Western blot, 细胞免疫荧光检测分析上调Twist1对E-cadherin表达的影响; 划痕实验分析对细胞侵袭运动能力的影响; 三维培养观察Twist1对MCF-7细胞成血管能力的影响。 结果 MCF-7细胞过表达Twist1抑制细胞E-cadherin的表达, 并且导致其亚细胞定位异常, 细胞迁移运动能力增强, 细胞成血管能力增强。 结论 Twist1能抑制MCF-7细胞E-cadherin的表达, 并增强其侵袭迁移及血管生成能力。
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| 关 键 词： | Twist1 E-cadherin 乳腺癌 |
| 收稿时间： | 2013-01-07 |
Effect of Twist1 on E-cadherin expression and subcellular location of human breast carcinoma cell line |
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| Affiliation: | ①.Department of Pathology, Tianjin Medical University, Tianjin 300070, China②.Department of Pathology, Cancer Hospital, Tianjin Medical University, Tianjin 300060, China |
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| Abstract: | Objective This study aims to investigate the effect of Twist1 on human breast carcinoma E-cadherin. Methods pcDNA-Twist1 was transfected into human breast carcinoma cell line MCF-7.The expression of E-cadherin was detected via real-time polymerase chain reaction and immunofluorescence staining.Wound-healing assay was performed to examine the migration ability of cells, and the 3D culture was used to observe vasculogenic mimicry(VM) formation. Results MCF-7 cells overexpressing Twist1 resulted in the suppression of E-cadherin expression and abnormal subcellular location.Migration ability and VM formation were also enhanced. Conclusion Twist1 prevented E-cadherin expression as well as promoted migration ability and VM formation in human breast carcinoma cell line. |
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| Keywords: | Twist1 E-cadherin breast carcinoma |
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