| 乙酰半胱氨酸对角膜新生血管形成过程中瘦素和PEDF表达的调节 |
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| 引用本文： | 郝瑞霖,杨炜,张奕霞,罗远湘,张晓雪.乙酰半胱氨酸对角膜新生血管形成过程中瘦素和PEDF表达的调节[J].眼科新进展,2014,0(8):718-721. |
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| 作者姓名： | 郝瑞霖 杨炜 张奕霞 罗远湘 张晓雪 |
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| 作者单位： | 新疆维吾尔自治区石河子市，石河子大学医学院眼科13班 |
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| 摘 要： | 目的 观察大鼠碱烧伤后不同时期角膜组织病理学变化、瘦素和色素上皮衍生因子（ｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍｄｅｒｉｖｅｄｆａｃｔｏｒ，ＰＥＤＦ）的表达以及乙酰半胱氨酸对瘦素和ＰＥＤＦ表达的影响，探讨他们与角膜新生血管（ｃｏｒｎｅａｌｎｅｏｖａｓｃｕｌａｒｉｚａｔｉｏｎ，ＣＮＶ）的关系。方法 使用碱烧伤诱导大鼠ＣＮＶ模型，采用浸润１ｍｏｌ?Ｌ－１ＮａＯＨ溶液，直径为３．０ｍｍ的单片滤纸，准确置于大鼠右眼角膜中央２３ｓ制作ＣＮＶ模型。７５只大鼠随机分成３组，每组２５只，左眼为正常对照眼。Ａ组自烧伤之日起每天滴注ＰＢＳ液；Ｂ组每天滴注乙酰半胱氨酸；Ｃ组烧伤后７ｄ之内每天滴注ＰＢＳ液，７ｄ后每天加滴乙酰半胱氨酸。用裂隙灯显微镜观察ＣＮＶ，分别计算ＣＮＶ长度和面积，并行ＨＥ染色和免疫组织化学检测。结果 Ｂ组ＣＮＶ生长趋势与Ａ组、Ｃ组基本相似，但较Ａ组、Ｃ组生长迟缓，Ｃ组比Ａ组缓慢，但稍快于Ｂ组。Ｃ组角膜病理变化与Ａ组相似，较Ｂ组稍重。除４ｄ时Ａ组和Ｃ组间ＣＮＶ长度及面积差异均无统计学意义（均为Ｐ＞０．０５）外，余时间点组间两两比较差异均有统计学意义（均为Ｐ＜０．０５）。免疫组织化学检测显示角膜碱烧伤后，瘦素和ＰＥＤＦ的表达开始增高。伤后７ｄ，各组瘦素表达:Ａ组、Ｂ组、Ｃ组分别为０．２７６１±０１１４４、０２３２２±０．０７４９、０．２６９２±０．０６４６，ＰＥＤＦ表达分别为０．３５２１±０．１０７４、０．３８９９±０．０７２１、０．３５６１±０．１２３３，三组瘦素、ＰＥＤＦ表达总体比较差异有统计学意义（Ｐ＜０．０５）；两两比较显示，Ａ组与Ｂ组、Ｂ组与Ｃ组瘦素、ＰＥＤＦ表达差异均有统计学意义（均为Ｐ＜０．０５）。伤后１４ｄ，各组瘦素与ＰＥＤＦ的表达总体比较差异均有统计学意义（均为Ｐ＜０．０５）；各组间两两比较显示，瘦素、ＰＥＤＦ表达差异均有统计学意义（均为Ｐ＜０．０５）。结论 瘦素和ＰＥＤＦ表达水平与ＣＮＶ的生成具有相关性，乙酰半胱氨酸能有效降低瘦素的表达，提高ＰＥＤＦ的表达。
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| 关 键 词： | 碱烧伤 角膜新生血管 N-乙酰半胱氨酸 瘦素 色素上皮衍生因子 |
Regulative effects of acetylcysteine on leptin and PEDF in corneal neovascularization |
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| HAO Rui-Lin,YANG Wei,ZHANG Yi-Xia,LUO Yuan-Xiang,ZHANG Xiao-Xue.Regulative effects of acetylcysteine on leptin and PEDF in corneal neovascularization[J].Recent Advances in Ophthalmology,2014,0(8):718-721. |
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| Authors: | HAO Rui-Lin YANG Wei ZHANG Yi-Xia LUO Yuan-Xiang ZHANG Xiao-Xue |
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| Abstract: | Objective To observe the histopathological changes,leptin and pigment epithelium derived factor ( PEDF) expression. as well as effects of acetylcysteine on leptin and PEDF expression in different periods after corneal alkali burns. and explore their relationship with corneal neovascularization ( CNV) . Methods CNV models were established by putting the filter paper with the diameter of 3 mm immersed into I mol . L -l NAOH solution on the central corneas of right eyes of rats for 30 seconds. Right eyes of 75 rats were treated as experimental group and divided into groups A,B and C,25 eyes in each group. Left eyes were treated as normal control group. Starting from the date of the burn , group A were dropped with PBS , group B with acetylcysteine 5 times per day, and group C with PBS within 7 day, then with acetylcysteine 5 times a day from 7 days t0 14 days. CNV was observed by slit lamp microscope every day, and the length and area of CNV were calculated and detected by HE staining and immunohistochemical method. Results The growth tendency of CNV in group B was primary similar to group A and C,the growth rate of group B was lower than those of group A and C ,and group C was lower than group A. The corneal histopathological changes in group C were similar to group A,but more server than group B. Except for 4 days after alkali burn , there were statistical differences in the length and area of CNV at other time points between group A and C ( all P < 0. 05 ) . Immunohistochemical detection showed that,the expression of leptin and PEDF began to rise after corneal alkali burn. The expression of leptin at 7 days after alkali burn in group A,B and C were 0. 273 I +0. 114 4 . 0. 232 2 + 0. 074 9 and 0. 269 2 + 0. 064 6 , respectively, the expression of PEDF were 0. 352 I +0. 107 4 ,0. 389 9 +0. 072 I and 0. 356 I +0. 123 3 ,respectively , there was significant difference among three groups (P < 0. 05 ) . and there were statistical differences between group A and B , group B and C ( all P < 0. 05) . At 14 days after alkali burn , there was significant difference in the expression of leptin and PEDF among three groups(P < 0. 05 ) . and there were statistical differences between each two groups ( all P < 0. 05 ) . Conclusion Expressions of leptin and PEDF are related with CNV. Acetylcysteine can effectively reduce the expression of leptin , enhance the expression of PEDF. |
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| Keywords: | alkali burn corneal neovascularization N-acetylcysteine leptin pigment epithelium derived factor |
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