| 云南省狂犬病疫情实验室检测及病毒分子流行病学分析 |
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| 引用本文： | 亚红祥,王静林,张云智. 云南省狂犬病疫情实验室检测及病毒分子流行病学分析[J]. 疾病监测, 2014, 29(9): 737-740. DOI: 10.3784/j.issn.1003-9961.2014.09.017 |
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| 作者姓名： | 亚红祥 王静林 张云智 |
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| 作者单位： | 云南省地方病防治所/云南省病毒立克次体研究中心/云南省自然疫源性疾病防控技术重点实验室,云南大理671000 |
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| 基金项目： | 志谢：非常感谢大理、普洱、西双版纳、楚雄、德宏、玉溪、临沧等地区各级CDC相关疾控人员对样品的采集工作的帮助和支持. |
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| 摘 要： | ![]()
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| 关 键 词： | 狂犬病 狂犬病毒 实验室检测 流行病学 云南省 |
| 收稿时间： | 2014-05-14 |
Molecular epidemiological characteristics of rabies virus detected in Yunnan |
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| YA Hong-xiang,WANG Jing-lin,ZHANG Yun-zhi. Molecular epidemiological characteristics of rabies virus detected in Yunnan[J]. Disease Surveillance, 2014, 29(9): 737-740. DOI: 10.3784/j.issn.1003-9961.2014.09.017 |
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| Authors: | YA Hong-xiang WANG Jing-lin ZHANG Yun-zhi |
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| Affiliation: | . (Yunnan Provincial Institute of Endemic Disease Control and Prevention/Yunnan Center of Virus and Rickettsia Research/Yunnan Key Laboratory of Natural Focus Diseases, Dali 671000, Yunnan, China) |
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| Abstract: | ![]()
Objective To understand the molecular epidemiological characteristics of rabies in Yunnan province.Methods Brain tissue samples of animals and saliva samples of patients were collected,and the presence of rabies virus was determined by direct immuno fluorescence assay（DFA） and RT-PCR. Homology and phylogenetic analyses were performed with other known sequences. Results Twenty-six of 60 samples from sick animals and 2 of 182 samples from healthy dogs were confirmed to be antigen positive for rabies virus by DFA,and the saliva samples from 4 patients and brain samples from 73 rodents were confirmed to be negative for rabies virus by DFA. Among the 28 antigen positive samples,25 were confirmed to be nucleic acid positive for rabies virus by RT-PCR. Phylogenetie analysis indicated that DX01 and DZ01 strains in this study and some domestic rabies virus strains belonged to one branch,and DN01 strain in this study and some strains from Southeast Asia belonged to one branch. Conclusion The rabies viruses causing rabies in Yunnan were complex,which might be imported from other provinces in China and Southeast Asia. |
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| Keywords: | Rabies Rabies virus Laboratory tests Epidemiology Yunnan province |
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