Detection of enteroaggregative Escherichia coli with formalin-preserved HEp-2 cells |
| |
| Authors: | Miqdady Mohamad S Jiang Zhi-Dong Nataro James P DuPont Herbert L |
| |
| Affiliation: | Baylor College of Medicine, Houston, Texas, USA. |
| |
| Abstract: | ![]()
Formalin-stored HEp-2 cells were used to assay Escherichia coli for adherence. Cells refrigerated in formalin for up to 28 days and used in a wet assay format demonstrated an assay sensitivity ranging from 94 to 98% to detect enteroaggregative E. coli (EAEC). HEp-2 cells first fixed and stored with formalin and then stored dry in ambient conditions for 6 weeks demonstrated an assay sensitivity of 92% to detect EAEC. Using formalin-fixed HEp-2 cells will improve the efficiency of EAEC identification. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
