| 模拟失重对大鼠心肌细胞凋亡的影响 |
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| 引用本文： | 高原,孙喜庆,杜挺媛,石菲,张舒,王冰,王永春. 模拟失重对大鼠心肌细胞凋亡的影响[J]. 心脏杂志, 2012, 24(6): 673-676 |
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| 作者姓名： | 高原 孙喜庆 杜挺媛 石菲 张舒 王冰 王永春 |
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| 作者单位： | (第四军医大学航空航天医学系航空航天生物动力学教研室、航空航天医学教育部重点实验室，陕西 西安 710032) |
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| 基金项目： | 国家自然科学基金项目资助(30900279,81171872) |
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| 摘 要： | 目的:探讨模拟失重对体外培养的大鼠心肌细胞凋亡的影响。方法: 以原代培养的乳鼠心肌细胞为研究对象，以细胞回转器模拟失重效应，通过流式细胞检测技术观察模拟失重96 h对心肌细胞凋亡率的影响，进一步通过RT-PCR的方法，检测模拟失重96 h后，心肌细胞凋亡相关基因p53、bcl-2和caspase-3 mRNA表达的影响。结果: 模拟失重96 h可显著增高心肌细胞的凋亡率(P<0.05)，与对照组相比，回转器模拟失重可显著增高促凋亡基因p53、caspase-3 mRNA的表达(P<0.05)，而抑凋亡基因bcl-2 mRNA则表达显著减低(P<0.05)。结论: 回转器模拟失重96 h致心肌细胞凋亡表达增高，其可能机制是凋亡相关基因表达失衡有关。
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| 关 键 词： | 模拟失重 心肌细胞 大鼠 细胞凋亡 |
| 收稿时间： | 2012-06-27 |
Effects of simulated microgravity on rat cardiomyocyte apoptosis |
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| GAO Yuan,SUN Xi-qing,DU Ting-yuan,SHI Fei,ZHANG Shu,WANG Bing,WANG Yong-chun. Effects of simulated microgravity on rat cardiomyocyte apoptosis[J]. Chinese Heart Journal, 2012, 24(6): 673-676 |
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| Authors: | GAO Yuan SUN Xi-qing DU Ting-yuan SHI Fei ZHANG Shu WANG Bing WANG Yong-chun |
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| Affiliation: | (Department of Aerospace Biodynamics,School of Aerospace Medicine,Fourth Military Medical University,Xi’ an 710032,Shaanxi,China) |
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| Abstract: | AIM:To investigate the effects of simulated microgravity on apoptosis of neonatal primary rat cardiomyocytes. METHODS: Clinostat was used to simulate microgravity condition. Apoptosis rates were examined by flow cytometry, and mRNA expression of p53, bcl-2 and caspase-3 were measured by RT-PCR methods. RESULTS: Flow cytometry results showed that the rates of apoptotic cardiomyocytes were higher than those in control group (P<0.05). Compared with those in control group, mRNA expression of p53 and caspase-3 increased significantly (P<0.05), whereas bcl-2 mRNA expression decreased significantly (P<0.05). CONCLUSION: Simulated microgravity induces apoptosis of neonatal primary rat cardiomyocytes. |
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